Multimodal Optical Coherence Tomography in Visualization of Brain Tissue Structure at Glioblastoma (Experimental Study)
| Autor: | V. V. Fedoseeva, М.М. Karabut, A.S. Khalansky, S.S. Kuznetsov, Vadim Elagin, К.S. Yashin, Natalia D. Gladkova, I.А. Medyanik, L.Ya. Kravets, Е.B. Kiseleva, Lev A. Matveev |
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| Jazyk: | ruština |
| Rok vydání: | 2016 |
| Předmět: |
medicine.diagnostic_test
genetic structures Computer science General Medicine medicine.disease General Biochemistry Genetics and Molecular Biology eye diseases Visualization Optical coherence tomography medicine Brain tissue structure sense organs optical coherence tomography microangiographic OCT glioblastoma cross-polarization OCT multimodal OCT experimental model of tumor 101.8 rat glioblastoma intraoperative diagnosis Glioblastoma Biomedical engineering |
| Zdroj: | ResearcherID |
| ISSN: | 2076-4243 |
| Popis: | The aim of the investigation was to evaluate the performance of multimodal OCT (MM OCT) for differential diagnostics of normal and diseased brain tissue using an experimental model of glioblastoma. Materials and Methods. The spectral domain MM OCT device developed at the Institute of Applied Physics of the Russian Academy of Sciences (Nizhny Novgorod, Russia) was used for the study. It provides two modes of investigation: cross-polarization OCT (CP OCT) and microangiographic OCT (MA OCT). The instrument features the following characteristics: rate of information gathering — 20,000 A-scans per second; wavelength — 1.3 μm; shot size — ~4×2 mm; lateral resolution — 20 μm; axial resolution — 10–15 μm. The OCT investigation was performed on an experimental 101.8 rat brain glioblastoma tumor model inoculated and maintained in the Research Institute of Human Morphology. To evaluate the signal parameters typical of the tumor and of normal brain tissue, CP OCT and MA OCT images were compared with histological specimens (stained with hematoxylin and eosin). Analysis of the MA OCT images was performed on the basis of comparison with the findings of ZOOM-microscopy. Results. The model of the rat 101.8 glioblastoma helped to identify links between CP OCT images of areas of brain tissue and their morphological structure. We performed a comparative evaluation of the signals from the glial tumor and from normal brain tissue. MA OCT allowed the visualization of the blood vessels both in the tumor and in the normal brain tissues, revealing changes in the form and sizes typical of the tumor vessels. Conclusion. ММ OCT is an innovative technology with potential for use in intraoperational diagnoses of glial tumors of the brain. The ability to combine several modes of investigation enables information to be obtained simultaneously about the structure of the tissues and about any peculiarities of the structure of the different elements of their microvascular network. |
| Databáze: | OpenAIRE |
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