Chemotherapeutic potentials of the stem bark of Balanite aegyptiaca (L.) Delile: an antiangiogenic, antitumor and antioxidant agent
| Autor: | Amin Malik Shah Abdul Majid, Nagla Mustafa Eltayeb, Saad S. Dahham, Aman Shah Abdul Majid, Loiy Elsir Ahmed Hassan, Sultan A M Saghir, Abdelhafeez M. A. Mohammed |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male Antioxidant DPPH Angiogenesis medicine.medical_treatment Cytotoxicity Mice Nude Angiogenesis Inhibitors Pharmacology complex mixtures Antioxidants Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine In vivo Cell Movement Cell Line Tumor medicine Human Umbilical Vein Endothelial Cells Animals Humans Balanites Aorta Cell Proliferation Tube formation ABTS business.industry Plant Extracts Growth factor General Medicine Antitumor Rats 030104 developmental biology Complementary and alternative medicine chemistry 030220 oncology & carcinogenesis Antiangiogenic MCF-7 Cells Plant Bark Nude mice Balanite aegyptiaca business Research Article |
| Zdroj: | BMC Complementary and Alternative Medicine |
| ISSN: | 1472-6882 |
| Popis: | Background Balanite aegyptiaca (L.) Delile, is a plant with extensive medicinal properties. Its stem bark is traditionally known for its spasmolytic and antiepileptic properties and used to treat yellow fever, jaundice and syphilis. Angiogenesis (sprouting of new blood vessels) is crucial for tumor growth and metastasis. The goal of this study is investigate the antiangiogenic, cytotoxicity and antioxidant activity as well as antitumor in vivo properties of B. aegyptiaca stem bark extracts. Method The dried powder of stem bark was extracted sequentially with n-hexane, chloroform, methanol and water. Rat aorta ring assay (RARA) was used as a platform to screen for antiangiogenic affect. The most active extract was subjected to further confirmatory antiangiogenic tests i.e. cell migration, tube formation and VEGF inhibition and finally evaluated for its in vivo antitumor efficacy in nude mice. The cytotoxicity of extracts on four cancer cell lines (HCT-116, K562, U937 and MCF-7) and one normal cells line (HUVEC) was evaluated. To assess the antioxidant activity screening, four methods were used, (DPPH•) and ABTS radical scavenging activity, as well as total flavonoids and phenolic contents. Results Methanol extract of B. aegyptiaca stem bark (MBA) showed the highest antiangiogenic, antioxidant and anticancer properties. It was found selectively cytotoxic to leukemia cell lines as well as breast cancer cell line MCF-7. (MBA) thus exhibited antiangiogenic in ex-vivo rat aorta ring model; it was found to excel its antiangiogenic effect via inhibition of the key growth factor (VEGF) as well as to halt HUVEC cell migration and tube formation, furthermore animals bearing colon cancer treated with (MBA) showed significant reduction in tumor growth. Conclusion Different extracts of B. aegyptiaca stem bark showed various anticancer and antiangiogenic properties. MBA demonstrated potent antiangiogenic, antioxidant and antitumor in vivo. The outcome of this study suggests the potential of stem bark of the B. aegyptiaca for developing chemotherapeutic agent against solid tumor as well as leukemia. |
| Databáze: | OpenAIRE |
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