Microsatellite sequences in a conifer,Pinus sylvestris
| Autor: | Pertti Pulkkinen, Pekka Vakkari, Silja Kostia, Sirkka-Liisa Varvio |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
0106 biological sciences
DNA Plant Sequence analysis Molecular Sequence Data Plant genetics medicine.disease_cause Polymerase Chain Reaction 01 natural sciences law.invention 03 medical and health sciences law Pollen Genetics medicine Genomic library Molecular Biology Polymerase chain reaction 030304 developmental biology Base Composition 0303 health sciences Polymorphism Genetic Base Sequence biology Gene Amplification Scots pine Pinus sylvestris Sequence Analysis DNA General Medicine biology.organism_classification DNA Fingerprinting DNA profiling Microsatellite Microsatellite Repeats 010606 plant biology & botany Biotechnology |
| Zdroj: | Genome. 38:1244-1248 |
| ISSN: | 1480-3321 0831-2796 |
| DOI: | 10.1139/g95-163 |
| Popis: | Scots pine (Pinus sylvestris) genomic libraries were constructed and screened with oligonucleotide probes (GT)10, (CT)10, and (AT)10. Eight microsatellites were identified from 6000 clones screened. The longest microsatellite stretch found, (GT)9(N)21(AT)24, was amplified from bud and single pollen grain samples. In order to clarify the complex amplification pattern revealed, two PCR products were sequenced. The size differences were caused both by varying repeat numbers of the microsatellite stretches and by differences in other parts of the amplified sequence. This kind of complex molecular basis of microsatellite amplification within a species has not been previously reported. Microsatellite sequences were used as PCR primers to detect polymorphisms and to estimate the abundance of microsatellites.Key words: microsatellites, Pinus sylvestris, plant genome, inter SSR–PCR. |
| Databáze: | OpenAIRE |
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