Insights into in vivo adipocyte differentiation through cell-specific labeling in zebrafish
| Autor: | Jose L. Badano, Leonel Malacrida, Paola Lepanto, Uriel Koziol, Florencia Levin-Ferreyra |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
QH301-705.5
Adipose Tissue White Transgene Science Cell White adipose tissue Fatty Acid-Binding Proteins adipocyte General Biochemistry Genetics and Molecular Biology Cell Line Animals Genetically Modified blood vessels chemistry.chemical_compound In vivo Adipocyte Adipocytes medicine Animals Biology (General) Zebrafish Adipogenesis biology nile red Endothelial Cells Cell Differentiation biology.organism_classification zebrafish In vitro Cell biology medicine.anatomical_structure chemistry CCAAT-Enhancer-Binding Proteins Complement Factor D Adiponectin General Agricultural and Biological Sciences Research Article |
| Zdroj: | Biology Open, Vol 10, Iss 9 (2021) Biology Open article-version (VoR) Version of Record |
| ISSN: | 2046-6390 |
| Popis: | White adipose tissue hyperplasia has been shown to be crucial for handling excess energy in healthy ways. Though adipogenesis mechanisms have been underscored in vitro, we lack information on how tissue and systemic factors influence the differentiation of new adipocytes. While this could be studied in zebrafish, adipocyte identification currently relies on neutral lipid labeling, thus precluding access to cells in early stages of differentiation. Here we report the generation and analysis of a zebrafish line with the transgene fabp4a(-2.7):EGFPcaax. In vivo confocal microscopy of the pancreatic and abdominal visceral depots of transgenic larvae, revealed the presence of labeled mature adipocytes as well as immature cells in earlier stages of differentiation. Through co-labeling for blood vessels, we observed a close interaction of differentiating adipocytes with endothelial cells through cell protrusions. Finally, we implemented hyperspectral imaging and spectral phasor analysis in Nile Red-labeled transgenic larvae and revealed the lipid metabolic transition towards neutral lipid accumulation of differentiating adipocytes. Altogether our work presents the characterization of a novel adipocyte-specific label in zebrafish and uncovers previously unknown aspects of in vivo adipogenesis. This article has an associated First Person interview with the first author of the paper. Summary: Analysis of the differentiation of adipocytes in vivo through cell-specific labeling in zebrafish, revealed their early interaction with blood vessels as well as early lipid metabolic changes. |
| Databáze: | OpenAIRE |
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