OPTIMAL PARAMETERS FORT In Vitro DEVELOPMENT OF THE HYDROCARBONOCLASTIC MICROORGANISM Proteus sp
| Autor: | Miguel A. Hernández-Rivera, Y Córdova-Bautista, V.M Villegas-Cornelio, J.G Martínez-Vázquez, Marcia E. Ojeda-Morales |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Journal of soil science and plant nutrition, Volume: 11, Issue: 1, Pages: 29-43, Published: 2011 Journal of soil science and plant nutrition v.11 n.1 2011 SciELO Chile CONICYT Chile instacron:CONICYT |
| Popis: | In México, there are extensive areas polluted by oil spills. Currently, bioremediation technologies have been developed, using microorganisms to clean up oil sites. In this study, we evaluated the development of a hydrocarbonoclastic bacterial strain, using a completely randomized 3x3x4 factor arrangement: three temperatures, three pH, and four nutrients. We collected samples of soil contaminated with 3.45 x 10(5) mg kg-1 (345,000 ppm) Total Petroleum Hydrocarbons (TPH), located at the Ejido José N. Rovirosa, Huimanguillo, Tabasco, México. The samples were grown in a culture medium Nutrient Agar (NA), obtaining a bacterial strain, which was characterized and classified as Proteus sp. The strain was grown in a combined carbon culture medium and then in a liquid mineral medium with crude oil as sole carbon source. Analysis of variance and mean test were performed, using the SPSS-11.0 statistical software. The microorganisms showed the highest population growth in the treatment N2=Triple17pH1=4.029°C with a value of 4.6 x 10(10) CFU mL-1. To reach, by bioaugmentation, the same development of Proteus sp in a conditioned soil would allow us to implement a potential bioremediation strategy for solving the problem of soils contaminated with hydrocarbons in the state of Tabasco in particular, and in Mexico in general. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|