Immunolocalization of Sonic Hedgehog (Shh) in Developing Mouse Lung
| Autor: | Susan E. Wert, Leigh-Anne D. Miller, Jeffrey A. Whitsett |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty animal structures Histology Morphogenesis Respiratory Mucosa Mice 03 medical and health sciences Tubulin medicine Animals Uteroglobin Hedgehog Proteins Sonic hedgehog Lung 030102 biochemistry & molecular biology biology Cilium Proteins Forkhead Transcription Factors respiratory system Immunohistochemistry Embryonic stem cell Epithelium respiratory tract diseases Cell biology DNA-Binding Proteins 030104 developmental biology medicine.anatomical_structure Animals Newborn embryonic structures Trans-Activators biology.protein Lung morphogenesis Anatomy Immunostaining |
| Zdroj: | Journal of Histochemistry & Cytochemistry. 49:1593-1603 |
| ISSN: | 1551-5044 0022-1554 |
| Popis: | Expression of sonic hedgehog (Shh) is required for normal development of the lung during embryogenesis. Loss of Shh expression in mice results in tracheoesophageal fistula, lung hypoplasia, and abnormal lung lobulation. To determine whether Shh may play a role later in lung morphogenesis, immunostaining for Shh was performed in mouse lung from embryonic day (E) 10.5 to postnatal day (PD) 24. Shh was detected in the distal epithelium of the developing mouse lung from E10.5 to E16.5. From E16.5 until PD15, Shh was present in epithelial cells in both the peripheral and conducting airways. Although all cells of the developing epithelium uniformly expressed Shh at E10.5, Shh expression was restricted to subsets of epithelial cells by E16.5. Between E16.5 and PD15, non-uniform Shh staining of epithelial cells was observed in the conducting airways in a pattern consistent with the distribution of non-ciliated bronchiolar cells (i.e., Clara cells) and the Clara cell marker CCSP. Shh did not co-localize with hepatocyte nuclear factor/forkhead homologue-4 (HFH-4), β-tubulin, or with the presence of cilia. These results support the concept that Shh plays a distinct regulatory role in the lung later in morphogenesis, when it may influence formation or cytodifferentiation of the conducting airways. |
| Databáze: | OpenAIRE |
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