Mitogenic potentials of bestatin, amastatin, arphamenines A and B, FK-156 and FK-565 on spleen lymphocytes
| Autor: | Norbert Weibmann, Gabriele Leyhausen, Armin Maidhof, Wataru Tanaka, Hamao Umezawa, Werner E.G. Müller |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Male
medicine.medical_specialty Cell Peptide Spleen Biology Diaminopimelic Acid Glutamyl Aminopeptidase Lymphocyte Activation Aminopeptidases Aminopeptidase Leucyl Aminopeptidase Mice chemistry.chemical_compound Amastatin Adjuvants Immunologic Leucine Amphotericin B Internal medicine Drug Discovery medicine Animals Lymphocytes Pharmacology chemistry.chemical_classification Amino Acids Diamino Dipeptides Molecular biology Anti-Bacterial Agents Cytosol medicine.anatomical_structure Endocrinology Enzyme chemistry Microsome Mitogens Peptides Oligopeptides |
| Zdroj: | The Journal of Antibiotics. 38:772-778 |
| ISSN: | 1881-1469 0021-8820 |
| DOI: | 10.7164/antibiotics.38.772 |
| Popis: | The following aminopeptidase (AP) activities were found to be associated with the surface of mouse spleen cells: Leu-AP (138 pmol/10(5) cells X minute) and AP-B (16 pmol/10(5) cells X minute with Lys-beta-naphthylamide as substrate and 21 pmol/10(5) cells X minute with Arg-beta-naphthylamide substrate); AP-A activity was not detected by the assay system applied. The immunoactive peptide bestatin inhibited the Leu-AP, while AP-B activity decreased in the presence of both arphamenines A and B and bestatin. No effects on these enzymes were caused by amastatin (an AP-A inhibitor), FK-156, FK-565 and Bu-2743E; the latter peptide turned out to be not an inhibitor of cell surface associated microsomal Leu-AP but an inhibitor of cytosolic Leu-AP. The immunoactive peptides bestatin, arphamenines A and B, and amastatin increased [3H]thymidine incorporation into spleen cells containing lymphocytes and macrophages. These mitogenic actions were not observed when macrophages were removed from the cultures or the cells had been stimulated with ConA or LPS. The lactoyl- and heptanoyl peptides FK-156 and FK-565 caused a mitogenic action on lymphocytes independently of the presence of macrophages. The inhibitor of cytosolic Leu-AP did not change the incorporation into lymphocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|