Direct DNA isolation from solid biological sources without pretreatments with proteinase-K and/or homogenization through automated DNA extraction
| Autor: | Joon Yong Yoon, Bong Youb Lee, Gi Young Jang, Jang-Seu Ki, Ki Byum Chang, Hee June Roh |
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| Rok vydání: | 2007 |
| Předmět: |
DNA
Bacterial Magnetic Bead Technology DNA Plant Biocompatible Materials Bioengineering Applied Microbiology and Biotechnology Extractor Automation Magnetics chemistry.chemical_compound Base sequence DNA Primers Chromatography Base Sequence biology Chemistry DNA Equipment Design Plants Proteinase K DNA extraction genomic DNA biology.protein Endopeptidase K Biotechnology Homogenization (biology) |
| Zdroj: | Journal of Bioscience and Bioengineering. 103:242-246 |
| ISSN: | 1389-1723 |
| DOI: | 10.1263/jbb.103.242 |
| Popis: | Genomic DNA from solid biomaterials was directly isolated with an automated DNA extractor, which was based on magnetic bead technology with a bore-mediated grinding (BMG) system. The movement of the bore broke down the solid biomaterials, mixed crude lysates thoroughly with reagents to isolate the DNA, and carried the beads to the next step. The BMG system was suitable for the mechanical homogenization of the solid biomaterials and valid as an automated system for purifying the DNA from the solid biomaterials without the need for pretreatment or disruption procedures prior to the application of the solid biomaterials. |
| Databáze: | OpenAIRE |
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