Protective responses of two paralogs of granulocyte colony stimulating factor (GCSF) in rock bream, Oplegnathus fasciatus during bacterial and viral infection
Autor: | Jae-Dong Shim, Jin-Sol Bae, Chan-Il Park, Ji-Min Jeong, Joseph Jeswin |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Fish Proteins DNA Complementary Transcription Genetic medicine.medical_treatment Spleen Aquatic Science 03 medical and health sciences Fish Diseases Streptococcal Infections Gene expression Granulocyte Colony-Stimulating Factor medicine Environmental Chemistry Animals Streptococcus iniae Amino Acid Sequence RNA Messenger Edwardsiella tarda Phylogeny Kidney biology Enterobacteriaceae Infections General Medicine biology.organism_classification DNA Virus Infections Granulocyte colony-stimulating factor Iridoviridae Perciformes Haematopoiesis 030104 developmental biology Cytokine medicine.anatomical_structure Immunology Sequence Alignment |
Zdroj: | Fishshellfish immunology. 65 |
ISSN: | 1095-9947 |
Popis: | Granulocyte colony stimulating factor (GCSF) has a key role in the production of neutrophilic granulocytes during normal hematopoietic development and release of neutrophils into the blood circulation. In this study we have identified and characterized two paralogs of GCSF (RbGCSF) in rock bream. Although RbGCSF-1 and RbGCSF-2 share low sequence conservation, its domains and protein structure still share significant similarity. Basal levels of RbGCSF-1 gene expression was high in peripheral blood leukocytes (PBLs), spleen and intestine whereas the RbGCSF-2 was highly expressed in PBLs and kidney, of healthy animals. A significant induction of RbGCSFs were observed after the challenge with Streptococcus iniae in kidney, spleen and gills during initial hours of infection. Whereas Edwarsiella tarda infection caused a reasonable expression in kidney. Red seabream iridovirus caused induction of RbGCSF-1 transcription only in gills during initial hours. This higher expression of RbGCSF in early hours may be its response to induce emergency hematopoiesis, due to shortage of neutrophils to combat the surge in pathogens. The difference in induction of RbGCSF paralogs during infection may constitute to its different roles or overlapping functions. |
Databáze: | OpenAIRE |
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