Structural and functional characterization of aDictyostetiumgene encoding a DIF inducible, prestalk-enriched mRNA sequence
| Autor: | Jeffrey G. Williams, Adriano Ceccarelli, K A Jermyn, A. Early, S.J. McRobbie, Karen T. Duffy |
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| Rok vydání: | 1987 |
| Předmět: |
Regulation of gene expression
Signal peptide Messenger RNA Fungal protein Genes Fungal Molecular Sequence Data fungi DNA Recombinant RNA Fungal Ketones Biology Molecular biology Fungal Proteins Hexanones Transmembrane domain Gene Expression Regulation Sequence Homology Nucleic Acid Gene expression Genetics Dictyostelium Amino Acid Sequence RNA Messenger Peptide sequence Gene Repetitive Sequences Nucleic Acid |
| Zdroj: | Nucleic Acids Research. 15:7463-7476 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/15.18.7463 |
| Popis: | The pDd56 mRNA sequence is highly enriched in prestalk over prespore cells and is inducible by DIF, the putative Dictyostelium stalk-specific morphogen. We show that the pDd56 gene is composed of forty one copies of a twenty four amino acid, cysteine rich repeat. This is highly homologus to a repeat which we have previously shown to compose the major fraction of the pDd63 mRNA, another DIF inducible, prestalk-enriched sequence. The predicted pDd56 protein contains a putative signal peptide but does not appear to contain a transmembrane segment. In combination these features suggest it to be an extrinsic protein and we confirm this elsewhere by showing that the pDd56 gene encodes a known, extracellular protein of the stalk. The pDd56 mRNA is dependent upon exogenous DIF for its accumulation. We show that this control is exerted at the transcriptional level and that a restriction fragment containing 1.7Kb of upstream sequence directs temporally-regulated expression of the gene. |
| Databáze: | OpenAIRE |
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