In vitro study of lipid metabolism in the mealworm larval fat body
| Autor: | Ahmed Bahjou, J. Dutrieu, R. Moreau, Jacques Puiroux, L. Gourdoux |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Mealworm
medicine.medical_specialty Physiology 030310 physiology [SDV]Life Sciences [q-bio] Biochemistry 03 medical and health sciences chemistry.chemical_compound Internal medicine Hemolymph medicine Lipolysis Adipokinetic hormone ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences biology Lipid metabolism General Medicine Metabolism biology.organism_classification Trehalose Endocrinology chemistry Insect Science Lipogenesis |
| Zdroj: | Archives of Insect Biochemistry and Physiology Archives of Insect Biochemistry and Physiology, Wiley, 1990, 15, pp.21-32 |
| ISSN: | 0739-4462 1520-6327 |
| Popis: | Lipid metabolism in Tenebrio larval fat body has been studied in vitro. Lipid release required the presence of diluted hemolymph in the incubation medium. This time-dependent release of lipid was strongly stimulated in a dose-dependent manner by Tenebrio corpora cardiaca (CC) extracts or synthetic adipokinetic hormone (AKH I). Furthermore, some glycerol was released when larval fat body was incubated without hemolymph, and this phenomenon was also dose dependent for added CC extracts. Lipid synthesis was estimated in vitro by following the incorporation of radioactivity from [6-14C] glucose into fatty acids. Lipogenesis occurred in the absence of added carbohydrates in the medium, but it was stimulated by the addition of glucose, and especially trehalose (10 mg ml−1). Intestinal insulin-like peptide (ILP) also stimulated in vitro lipogenesis in a dose-dependent fashion. We conclude that lipolytic and lipogenetic activities of larval mealworm fat body in vitro are effectively under hormonal control. |
| Databáze: | OpenAIRE |
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