High voltage electric pulses efficiently induce fusion of cells in monolayer culture
| Autor: | Tadashi Eguchi, Kanji Ishizaki, Hae Ryong Chang, Mitou Ikenaga |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Xeroderma pigmentosum
DNA Repair Physiology DNA repair Biology Hybrid Cells Electrofusion Cell Fusion chemistry.chemical_compound Electricity medicine Animals Humans Fibroblast Molecular Biology Cells Cultured Xeroderma Pigmentosum Cell fusion Genetic Complementation Test Cell Biology General Medicine Anatomy DNA medicine.disease Complementation medicine.anatomical_structure chemistry Cell culture Biophysics |
| Zdroj: | Cell structure and function. 14(2) |
| ISSN: | 0386-7196 |
| Popis: | High voltage electric pulses, 1000 V/cm, were found to induce cell fusion efficiently when delivered to cells growing in a monolayer culture. The maximum yield of fused cells was about 30% of cells remaining after treatment. Colonies of interspecies hybrid cells between mouse and others also appeared with a frequency of 3 X 10(-4) - 2 X 10(-5) after culturing fused cells in a selection medium that permits the growth of only hybrid cells. This method of electrofusion was applied for complementation analysis of DNA repair-deficient xeroderma pigmentosum cells. Efficient cell fusion was also observed with these human diploid fibroblasts and the resulting heterodikaryons showed a recovery of ultraviolet light-induced unscheduled DNA synthesis to the same extent as in normal cells. |
| Databáze: | OpenAIRE |
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