Effect of chlorogenic acid on the quorum-sensing system of clinically isolated multidrug-resistant Pseudomonas aeruginosa
Autor: | Weiliang Zeng, Xiu-cai Zhang, Lingbo Wang, Tieli Zhou, Mo Shen, Yao Sun, Wenya Xu, Cui Zhou |
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Rok vydání: | 2021 |
Předmět: |
Virulence Factors
Virulence medicine.disease_cause Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Mice Pyocyanin Bacterial Proteins medicine Animals biology Chemistry Pseudomonas aeruginosa Elastase Biofilm Quorum Sensing General Medicine biology.organism_classification Anti-Bacterial Agents Multiple drug resistance Quorum sensing RAW 264.7 Cells Biofilms Chlorogenic Acid Bacteria Biotechnology |
Zdroj: | Journal of applied microbiologyREFERENCES. 132(2) |
ISSN: | 1365-2672 |
Popis: | Aims Quorum sensing (QS) is the intercellular communication used by bacteria to regulate collective behavior. QS regulates the production of virulence factors in many bacterial species and is considered to be an attractive target for reducing bacterial pathogenicity. Chlorogenic acid (CA) is abundant in vegetables, fruits, and traditional Chinese medicine, and has multiple activities. This study, aimed to investigated the QS quenching activity of CA against clinically isolated multidrug-resistant Pseudomonas aeruginosa. Methods and results The results showed that CA inhibited the mobility of bacteria, reduced the production of pyocyanin, and inhibited the activity of elastase. Furthermore, crystal violet staining and scanning electron microscope experiments showed that CA inhibited the formation of multidrug-resistant P. aeruginosa biofilm. CA at or below the concentration of 2560 µg/mL exerted negligible cytotoxicity to RAW264.7 cells. The study also examined the expression of QS-related genes, including lasI, lasR, rhlI, rhlR, pqsA, and pqsR in P. aeruginosa and found that the expression of these genes was down-regulated under CA treatment. Conclusions The study showed that CA could be used as an anti-virulence factor for treating clinical P. aeruginosa infection. Significance and impact of study For the first time, this study took clinically isolated multi-drug resistant P. aeruginosa as the experimental object, and suggested that CA might be an effective antimicrobial compound targeting QS in treating P. aeruginosa infection, thus providing a new therapeutic direction for treating bacterial infection and effectively alleviating bacterial resistance. |
Databáze: | OpenAIRE |
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