Metabolic profiling of wheat rachis node infection by Fusarium graminearum - decoding deoxynivalenol-dependent susceptibility
| Autor: | Jörg Bormann, Jakob Bönnighausen, Nicolas Schauer, Wilhelm Schäfer |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Fusarium Physiology Mutant Plant Science Biology 01 natural sciences Microbiology Cell wall 03 medical and health sciences chemistry.chemical_compound Sugar Alcohols Cell Wall Plant defense against herbivory Jasmonate Amino Acids Mycotoxin Pathogen Triticum gamma-Aminobutyric Acid Plant Diseases food and beverages Metabolism Mycotoxins biology.organism_classification 030104 developmental biology chemistry Host-Pathogen Interactions Mutation Metabolome Reactive Oxygen Species Trichothecenes 010606 plant biology & botany |
| Zdroj: | New Phytologist. 221:459-469 |
| ISSN: | 0028-646X |
| DOI: | 10.1111/nph.15377 |
| Popis: | Fusarium graminearum is a filamentous ascomycete and the causal agent of Fusarium head blight on wheat that threatens food and feed production worldwide as infection reduces crop yield both quantitatively by interfering with kernel development and qualitatively by poisoning any remaining kernels with mycotoxins. In wheat, F. graminearum infects spikelets and colonizes the entire head by growing through the rachis node at the bottom of each spikelet. Without the mycotoxin deoxynivalenol (DON), the pathogen cannot penetrate the rachis node and wheat is able to resist colonization. Using a global metabolite profiling approach we compared the metabolic profile of rachis nodes inoculated with either water, the Fusarium graminearum wild-type or the DON-deficient ∆tri5 mutant. Extensive metabolic rearrangements mainly affect metabolites for general stress perception and signaling, reactive oxygen species (ROS) metabolism, cell wall composition, the tri-carbonic acid (TCA) cycle and γ-aminobutyric acid (GABA) shunt as well as sugar alcohols, amino acids, and storage carbohydrates. The results revealed specific, DON-related susceptibility factors. Wild-type infection resulted in an oxidative burst and the induction of plant programmed cell death, while spread of the DON-deficient mutant was blocked in a jasmonate (JA)-related defense reaction in concert with other factors. Hence, the ∆tri5 mutant is prone to defense reactions that are, in the case of a wild-type infection, not initiated. |
| Databáze: | OpenAIRE |
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