Tyrosine phosphorylation in psoriatic T cells is modulated by drugs that induce or improve psoriasis
| Autor: | G. Nussbaum, M Goos, H.M. Ockenfels, K. Mertins, S.N. Wagner, T. Schultewolter |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Male animal structures Time Factors Adolescent T-Lymphocytes Dermatology Protein tyrosine phosphatase Lithium Lymphocyte Activation Receptor tyrosine kinase Tacrolimus chemistry.chemical_compound Psoriasis medicine Humans Tyrosine Phosphorylation Phytohemagglutinins Cells Cultured Aged chemistry.chemical_classification biology Ethanol Tyrosine phosphorylation Chloroquine T lymphocyte Middle Aged Protein-Tyrosine Kinases medicine.disease Propranolol Enzyme chemistry Case-Control Studies Immunology Cancer research biology.protein Cyclosporine Irritants Female Immunosuppressive Agents |
| Zdroj: | Dermatology (Basel, Switzerland). 191(3) |
| ISSN: | 1018-8665 |
| Popis: | The induction of protein tyrosine kinases (PTKs) is known to be a key element in the activation of lymphocytes.Because immunologic mechanisms are important in the pathogenesis of psoriasis, we examined the time course of tyrosine-phosphorylated proteins (p-tyr) as a marker for cellular PTK activity in phytohemagglutinin (PHA)-stimulated T cells of psoriatic patients and healthy controls.PHA-stimulated T cells from both groups expressed peaks of p-tyr after 15 min and 4 h. In T cells from psoriatics, the 15-min peak was smaller but the 4-hour peak reached an enormous maximum, which was 270% higher than the basic p-tyr value. PHA-stimulated T cells were additionally treated with psoriasis-provoking drugs (lithium, chloroquine, propranolol and ethanol) and the two immunosuppressive drugs cyclosporin A and FK 506. Lithium and propranolol were able to increase the p-tyr level after 15 min in PHA-stimulated T cells from psoriatics in contrast to controls. Chloroquine and ethanol did not have a significant effect on T cells of both groups. CsA markedly diminished the phosphorylation of intracellular tyrosines in T cells of psoriatics and controls, whereas FK 506 diminished the p-tyr level in controls only slightly.We have characterized important differences in p-tyr phosphorylation activities of psoriatic T cells compared to controls. This could be a hint to explain the known abnormalities of psoriatic T cells. |
| Databáze: | OpenAIRE |
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