Hybridization method for quantitation of replicating polyoma DNA sequences
| Autor: | Unni Spaeren, Rolf Eliasson, Waldemar Waldeck |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
DNA Replication
Gel electrophoresis HpaII viruses Biophysics Nucleic Acid Hybridization DNA Restriction Enzymes Cell Biology Biology Biochemistry Genome Molecular biology DNA sequencing Deoxyribonucleoside Restriction enzyme chemistry.chemical_compound chemistry DNA Viral Methods Carbon Radioisotopes Polyomavirus Molecular Biology Nitrocellulose DNA |
| Zdroj: | Analytical Biochemistry. 96:378-383 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(79)90595-5 |
| Popis: | Polyoma DNA was cleaved with restriction endonuclease HpaII, the fragments were separated by gel electrophoresis and transferred in good yield to separate nitrocellulose filters by a modification of the procedure of E. M. Southern (1975, J. Mol. Biol.98, 503–517). The filters were then used in hybridization experiments to localize the isotope in different parts of the polyoma genome after in vitro incorporation of labeled deoxyribonucleoside triphosphates into the DNA. |
| Databáze: | OpenAIRE |
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