Guanine oxidation by electron transfer: one- versus two-electron oxidation mechanism
| Autor: | Adam Kupan, Christel Seguy, Geneviève Pratviel, Bernard Meunier, Sylvain Broussy, Aude Saulière |
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| Rok vydání: | 2005 |
| Předmět: |
Tris
Guanine Porphyrins Time Factors Free Radicals Riboflavin Electrons Photochemistry Biochemistry Adduct chemistry.chemical_compound Electron transfer Electrochemistry Organometallic Compounds Hydroxymethyl Molecular Biology Chromatography High Pressure Liquid Manganese Molecular Structure Oligonucleotide Organic Chemistry DNA chemistry Radical ion Molecular Medicine Oxidation-Reduction |
| Zdroj: | Chembiochem : a European journal of chemical biology. 7(1) |
| ISSN: | 1439-4227 |
| Popis: | The degeneracy of the guanine radical cation, which is formed in DNA by oxidation of guanine by electron transfer, was studied by a detailed analysis of the oxidation products of guanine on oligonucleotide duplexes and by labeling experiments. It was shown that imidazolone, the major product of guanine oxidation, is formed through a one-electron oxidation process and incorporates one oxygen atom from O2. The formation of 8-oxo-7,8-dihydroguanine by a two-electron oxidation process was a minor pathway. The two-electron oxidation mechanism was also evidenced by the formation of a tris(hydroxymethyl)aminomethane adduct. |
| Databáze: | OpenAIRE |
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