Delivery of pemetrexed by magnetic nanoparticles: design, characterization, in vitro and in vivo assessment
| Autor: | Didem Aksu, Şenay Hamarat Şanlıer, Eda Çapkın, Ilgın Kımız Geboloğlu, Özge Sarı, Güliz Ak |
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| Přispěvatelé: | Ege Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Nanoparticle 01 natural sciences Biochemistry chemistry.chemical_compound In vivo 010608 biotechnology medicine pemetrexed O-carboxymethyl chitosan non-small cell lung cancer 010405 organic chemistry Chemistry nanoparticle General Medicine equipment and supplies In vitro 0104 chemical sciences Cancer treatment Pemetrexed magnetic targeting Targeted drug delivery A549-luc-C8 Antifolate Cancer research Magnetic nanoparticles human activities Biotechnology medicine.drug |
| Popis: | Drug-loaded magnetic nanoparticles have been developed because of the advantages of specific drug targeting in cancer treatment. Pemetrexed (PEM) is a multi-targeting antifolate agent that is effective for the treatment of many cancers, for example, non-small cell lung cancer. Here, PEM loaded magnetic O-carboxymethyl chitosan (O-CMC) nanoparticles were prepared to deliver PEM on tumor tissue with an external magnetic field. The modification of chitosan to O-CMC was confirmed by FTIR analysis. Nanoparticle synthesis was performed via ionic gelation method. The diameter of magnetic O-CMC nanoparticles (MCMC) was found to be 130.1 ± 22.96 nm. After PEM loading, diameter was found to be 123.9 ± 11.42 nm. The drug release of PEM loaded MCMC (PMCMC) was slower in physiological medium than in acidic medium. A549-luc-C8 and CRL5807 cell lines were used for MTT test which showed that IC50 values of nanoparticles were lower than PEM. The antitumor efficiency of PMCMC in xenograft tumor model was examined with in vivo imaging system (IVIS) and caliper and with hematological analyses. In vivo studies revealed that PMCMC had targeted antitumor activity in A549-luc-C8-tumor-bearing mice compared to PEM. As a result, it was suggested that PMCMC have great potential for the treatment of non-small cell lung cancer. © 2019, © 2019 Taylor & Francis Group, LLC. Ege Üniversitesi: 2013 ILAM 002 We would like to thank Ege University Scientific Research Project Office, Ege ?niversitesi (Project ID: 2013 ILAM 002) for the financial support. We would like to thank Dursun Demiroz for drawing the non-chemical illustrations. |
| Databáze: | OpenAIRE |
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