Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies

Autor: Lucie Blahová, Andreu Blanquer, Lucie Bacakova, Ivana Nemcakova, Petr Ryšánek, Lenka Zajíčková
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Vascular smooth muscle
Polymers
bioactive coating
Nanofibers
02 engineering and technology
01 natural sciences
Muscle
Smooth
Vascular
lcsh:Chemistry
Mice
Plasma
chemistry.chemical_compound
Coated Materials
Biocompatible
Coating
Amines
lcsh:QH301-705.5
Cells
Cultured
Spectroscopy
010302 applied physics
chemistry.chemical_classification
Tissue Scaffolds
Photoelectron Spectroscopy
General Medicine
Polymer
Adhesion
polycaprolactone nanofibers
021001 nanoscience & nanotechnology
musculoskeletal system
amine plasma polymer
Computer Science Applications
Polycaprolactone
cardiovascular system
0210 nano-technology
Surface Properties
Polyesters
Myocytes
Smooth Muscle
engineering.material
Article
Catalysis
Inorganic Chemistry
substrate morphology
0103 physical sciences
Animals
Physical and Theoretical Chemistry
Molecular Biology
Macrophages
Organic Chemistry
Substrate (chemistry)
cell adhesion
Rats
RAW 264.7 Cells
cell proliferation
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Chemical engineering
Nanofiber
engineering
Cytokine secretion
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 24
International Journal of Molecular Sciences. 2020, vol. 21, issue 24, p. 1-24.
International Journal of Molecular Sciences, Vol 21, Iss 9467, p 9467 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21249467
Popis: Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market.
Databáze: OpenAIRE
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