Hormonal Regulation of Matrix Metalloproteinase 9/Gelatinase B Gene Expression in Rabbit Uterine Cervical Fibroblasts
| Autor: | Yo Mori, Akira Ito, Keisuke Imada, Hideaki Nagase, Mana Namiki, Takashi Sato |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Blotting Western Uterus Cervix Uteri Biology Gene Expression Regulation Enzymologic chemistry.chemical_compound Pregnancy Internal medicine medicine Animals Humans Collagenases RNA Messenger Cloning Molecular Fibroblast Cervix Cells Cultured Progesterone Metalloproteinase Interleukin Cell Biology General Medicine Fibroblasts Blotting Northern Hormones medicine.anatomical_structure Endocrinology Matrix Metalloproteinase 9 Reproductive Medicine chemistry In utero Phorbol Collagenase Female Rabbits Interleukin-1 medicine.drug |
| Zdroj: | Biology of Reproduction. 56:575-580 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod56.3.575 |
| Popis: | Since the degradation of uterine cervical extracellular matrix is an essential process in cervical ripening and dilatation at term, we examined the effect of pregnancy on the level of pro-matrix metalloproteinase 9 (proMMP-9)/progelatinase B in the rabbit uterine cervix and its regulation in uterine cervical fibroblasts. Uterine cervices shortly before term parturition contained high levels of proMMP-9 compared with those of nonpregnant rabbits. Uterine cervical fibroblasts prepared from rabbits at 23 days of gestation did not produce proMMP-9; in contrast, interleukin (IL)-1 alpha, IL-1 beta, or phorbol 12-myristate 13-acetate (PMA) induced proMMP-9 production along with an increase of proMMP-9 mRNA, and the IL-1-mediated induction of proMMP-9 was synergistically enhanced by PMA. On the other hand, physiological concentrations of progesterone suppressed IL-1-and/or PMA-mediated production of proMMP-9 in a dose-dependent manner. This suppressive effect of progesterone on proMMP-9 production was accompanied by a decrease in matrix metalloproteinase 9 (MMP-9) mRNA, indicating that progesterone down-regulates the production of proMMP-9 at the transcriptional level. These results indicate that in the rabbit uterine cervix, IL-1 alpha and IL-1 beta are effective inducers of the proMMP-9 production, and progesterone is a physiological suppressor. Thus, MMP-9 and progesterone are very likely to play essential roles in cervical ripening and dilatation in the rabbit, and the production of MMP-9 in the cervix is finely regulated during pregnancy. |
| Databáze: | OpenAIRE |
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