Cloning of a new cytokine that induces IFN-γ production by T cells
Autor: | Haruki Okamura, Hiroko Tsutsui, Toshinori Komatsu, Masuo Yutsudo, Akira Hakura, Tadao Tanimoto, Kakuji Torigoe, Takanori Okura, Yoshiyuki Nukada, Kazuko Hattori, Kenji Akita, Motoshi Namba, Fujimi Tanabe, Kaori Konishi, Shigeharu Fukuda, Masashi Kurimoto |
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Rok vydání: | 1995 |
Předmět: |
Lipopolysaccharides
Male DNA Complementary Lipopolysaccharide Kupffer Cells T-Lymphocytes medicine.medical_treatment Molecular Sequence Data Spleen law.invention Interferon-gamma Mice chemistry.chemical_compound law medicine Animals Amino Acid Sequence Propionibacterium acnes Cloning Molecular Gene Gram-Positive Bacterial Infections chemistry.chemical_classification Mice Inbred BALB C Multidisciplinary Base Sequence biology Liver Diseases Interleukin-18 Th1 Cells Molecular biology Recombinant Proteins Amino acid Killer Cells Natural medicine.anatomical_structure Cytokine chemistry biology.protein Recombinant DNA Cytokines Interleukin 18 Antibody |
Zdroj: | Nature. 378:88-91 |
ISSN: | 1476-4687 0028-0836 |
Popis: | The mechanism underlying the differentiation of CD4+ T cells into functionally distinct subsets (Th1 and Th2) is incompletely understood, and hitherto unidentified cytokines may be required for the functional maturation of these cells. Here we report the cloning of a recently identified IFN-gamma-inducing factor (IGIF) that augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids, which have no obvious similarities to any peptide in the databases. Messenger RNAs for IGIF and interleukin-12 (IL-12) are readily detected in Kupffer cells and activated macrophages. Recombinant IGIF induces IFN-gamma more potently than does IL-12, apparently through a separate pathway. Administration of anti-IGIF antibodies prevents liver damage in mice inoculated with Propionibacterium acnes and challenged with lipopolysaccharide, which induces toxic shock. IGIF may be involved in the development of Th1 cells and also in mechanisms of tissue injury in inflammatory reactions. |
Databáze: | OpenAIRE |
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