Exploring the exocellular fungal biopolymer botryosphaeran for laccase-biosensor architecture and application to determine dopamine and spironolactone
| Autor: | Gabriel Junquetti Mattos, João H. Coelho, Ana Paula Pires Eisele, Aneli M. Barbosa-Dekker, Elen Romão Sartori, Camila Farinha Valezi, Robert F.H. Dekker, Jéseka G. Schirmann |
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| Rok vydání: | 2019 |
| Předmět: |
Dopamine
02 engineering and technology Carbon nanotube Biosensing Techniques engineering.material Spironolactone 01 natural sciences Analytical Chemistry law.invention law Limit of Detection Voltammetry Electrodes Glucans Detection limit Laccase Chromatography Chemistry Nanotubes Carbon 010401 analytical chemistry Electrochemical Techniques 021001 nanoscience & nanotechnology Ascorbic acid Enzymes Immobilized 0104 chemical sciences Dielectric spectroscopy engineering Biopolymer 0210 nano-technology Biosensor |
| Zdroj: | Talanta. 204 |
| ISSN: | 1873-3573 |
| Popis: | Laccase was immobilized on a glassy carbon electrode layered with multi-walled carbon nanotubes using a film of botryosphaeran, a fungal (1 → 3)(1 → 6)-β-D-glucan. This novel biosensing platform was characterized by electrochemical impedance spectroscopy and scanning electron microscopy, and applied for the determination of dopamine. Experimental variables such as enzyme concentration, pH value and operational parameters of the electroanalytical technique were optimized. Using square-wave voltammetry, there was a linear dependence of peak current and dopamine concentration within the range of 2.99–38.5 μmol L −1 with a limit of detection of 0.127 μmol L −1 . The biosensor was successfully applied in the determination of dopamine in pharmaceutical injection and synthetic biological samples, and presented good selectivity even in the presence of uric acid and ascorbic acid, as well as other phenolic compounds. The different aspects regarding the operational stability of the laccase biosensor were evaluated, demonstrating good intra-day and inter-day repeatability, and long-storage stability. Furthermore, this biosensor was evaluated in the indirect determination of spironolactone by using the analytical signal of dopamine, presenting a limit of detection of 0.94 μmol L −1 . The results obtained in the analysis of spironolactone in commercial pharmaceutical samples were satisfactory. |
| Databáze: | OpenAIRE |
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