Positive and Negative Regulation of the Master Metabolic Regulator mTORC1 by Two Families of Legionella pneumophila Effectors
| Autor: | Daniel K. Nomura, Zhao-Qing Luo, Li Xu, Russell E. Vance, Roberto Zoncu, Jessica L. Counihan, Justin A. De Leon, Brian M. Castellano, Rosalie E. Lawrence, Kevin C. Barry, Christopher J. Nicolai, Jiazhang Qiu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Legionella 030106 microbiology Medical Physiology Regulator amino-acids mTORC1 Mechanistic Target of Rapamycin Complex 1 Legionella pneumophila General Biochemistry Genetics and Molecular Biology Article Microbiology 03 medical and health sciences Bacterial Proteins 2.1 Biological and endogenous factors Aetiology Pathogen lcsh:QH301-705.5 Lung chemistry.chemical_classification amino acids biology Effector Membrane Proteins Translation (biology) Pneumonia biology.organism_classification Amino acid Protein Transport T4SS 030104 developmental biology effector Infectious Diseases chemistry lcsh:Biology (General) Host-Pathogen Interactions Pneumonia & Influenza Biochemistry and Cell Biology biological phenomena cell phenomena and immunity Legionnaires' Disease Carrier Proteins Infection |
| Zdroj: | Cell reports, vol 21, iss 8 Cell Reports, Vol 21, Iss 8, Pp 2031-2038 (2017) |
| Popis: | Summary All pathogens must acquire nutrients from their hosts. The intracellular bacterial pathogen Legionella pneumophila , the etiological agent of Legionnaires' disease, requires host amino acids for growth within cells. The mechanistic target of rapamycin complex 1 (mTORC1) is an evolutionarily conserved master regulator of host amino acid metabolism. Here, we identify two families of translocated L. pneumophila effector proteins that exhibit opposing effects on mTORC1 activity. The Legionella glucosyltransferase (Lgt) effector family activates mTORC1, through inhibition of host translation, whereas the SidE/SdeABC (SidE) effector family acts as mTORC1 inhibitors. We demonstrate that a common activity of both effector families is to inhibit host translation. We propose that the Lgt and SidE families of effectors work in concert to liberate host amino acids for consumption by L. pneumophila . |
| Databáze: | OpenAIRE |
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