Molecular and physiological diversity of nicotinic acetylcholine receptors in the midbrain dopaminergic nuclei
| Autor: | Alban de Kerchove d'Exaerde, Ruby Klink, Jean-Pierre Changeux, Michele Zoli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Patch-Clamp Techniques
alpha7 Nicotinic Acetylcholine Receptor Nicotinic Antagonists -- administration & dosage Dopamine Substantia Nigra -- drug effects Nicotinic Antagonists Neurons -- drug effects Receptors Nicotinic Rats Sprague-Dawley Mice chemistry.chemical_compound Tyrosine 3-Monooxygenase -- genetics Receptors Nicotinic -- deficiency Nicotinic Agonists Nicotinic Antagonist Glutamate Decarboxylase -- metabolism gamma-Aminobutyric Acid Neurons Glutamate Decarboxylase Reverse Transcriptase Polymerase Chain Reaction General Neuroscience Sciences bio-médicales et agricoles Nicotinic Agonists -- administration & dosage Cell biology Substantia Nigra -- metabolism Substantia Nigra Ventral tegmental area medicine.anatomical_structure Nicotinic agonist Dopamine -- metabolism GABAergic medicine.drug addiction dopamine GABA nAChR Parkinson's disease substantia nigra ventral tegmental area Microinjections Tyrosine 3-Monooxygenase Glutamate Decarboxylase -- genetics Substantia nigra Receptors Nicotinic -- genetics In Vitro Techniques Biology gamma-Aminobutyric acid medicine Animals Neurons -- metabolism RNA Messenger ARTICLE Neurons -- cytology Acetylcholine receptor Methyllycaconitine Ventral Tegmental Area -- metabolism Ventral Tegmental Area -- drug effects Ventral Tegmental Area Receptors Nicotinic -- metabolism RNA Messenger -- metabolism Mice Mutant Strains Rats Protein Subunits nervous system chemistry Neurons -- classification Tyrosine 3-Monooxygenase -- metabolism gamma-Aminobutyric Acid -- metabolism Neuroscience |
| Zdroj: | The Journal of neuroscience, 21 (5 Scopus-Elsevier |
| Popis: | Nicotinic acetylcholine receptors (nAChRs) on dopaminergic (DA) and GABAergic (Gaba) projection neurons of the substantia nigra (SN) and ventral tegmental area (VTA) are characterized by single-cell RT-PCR and patch-clamp recordings in slices of rat and wild-type, beta2-/-, alpha4-/-, and alpha7-/- mice. The eight nAChR subunits expressed in these nuclei, alpha3-7 and beta2-4, contribute to four different types of nAChR-mediated currents. Most DA neurons in the SN and VTA express two nAChR subtypes. One is inhibited by dihydro-beta-erythroidine (2 microm), alpha-conotoxin MII (10 nm), and methyllycaconitine (1 nm) but does not contain the alpha7 subunit; it possesses a putative alpha4alpha6alpha5(beta2)(2) composition. The other subtype is inhibited by dihydro-beta-erythroidine (2 microm) and has a putative alpha4alpha5(beta2)(2) composition. Gaba neurons in the VTA exhibit a third subtype with a putative (alpha4)(2)(beta2)(3) composition, whereas Gaba neurons in the SN have either the putative (alpha4)(2)(beta2)(3) oligomer or the putative alpha4alpha6alpha5(beta2)(2) oligomer. The fourth subtype, a putative (alpha7)(5) homomer, is encountered in less than half of DA and Gaba neurons, in the SN as well as in the VTA. Neurons in the DA nuclei thus exhibit a diversity of nAChRs that might differentially modulate reinforcement and motor behavior. In Vitro Journal Article Research Support, Non-U.S. Gov't SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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