Stimulation and inhibition of DNA synthesis and cell growth by paraquat in cultured cells
| Autor: | Reiko Tanaka, Yasuji Amano |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Paraquat
Cell Stimulation Biology Toxicology chemistry.chemical_compound medicine Humans heterocyclic compounds Lung Mitosis Cells Cultured Dose-Response Relationship Drug DNA synthesis Colcemid Cell growth DNA Embryo Mammalian Molecular biology medicine.anatomical_structure Biochemistry chemistry Cell culture Autoradiography Cell Division Thymidine |
| Zdroj: | The Journal of Toxicological Sciences. 10:309-314 |
| ISSN: | 1880-3989 0388-1350 |
| DOI: | 10.2131/jts.10.309 |
| Popis: | Paraquat, a popular herbicide, is known to be very toxic to man and animals. Using cultured mammalian cells (human embryo lung cell, R-66), the effect of paraquat has been studied. With autoradiography, stimulation of DNA synthesis was observed when the cells were exposed to 0.08 or 0.4 microM of paraquat; however, DNA synthesis was inhibited with 40 microM of paraquat. To determine cell growth rate, accumulation of mitotic cells was determined after addition of colcemid (0.2 microgram/ml) into the culture medium. The ratios of mitotic cells observed were proportional to the level of DNA synthesis. And the stimulation and inhibition of cell growth were observed even after washing off paraquat from media. |
| Databáze: | OpenAIRE |
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