Biomechanical and functional properties of trophoblast cells exposed to Group B Streptococcus in vitro and the beneficial effects of uvaol treatment
| Autor: | Liliane Patricia G. Tenorio, Alexandre Urban Borbely, Samuel T. Souza, Rayane M. Botelho, Elaine Cristina Oliveira da Silva, Julie Bergeron, Marie-Julie Allard, Eduardo J. S. Fonseca, Josué Carinhanha Caldas Santos, Guillaume Sébire, Ana Lucia M. Silva, Karen S.C. Borbely, Keyla Pires, Camilla M. Gonçalves, Eloiza L.L. Tanabe, Aldilane Lays Xavier Marques |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Programmed cell death Cytoskeleton organization Cell Survival Biophysics Inflammation Biochemistry Cell Line Streptococcus agalactiae Andrology 03 medical and health sciences 0302 clinical medicine Pregnancy Placenta Chlorocebus aethiops medicine Animals Humans Secretion Viability assay Molecular Biology Vero Cells reproductive and urinary physiology Nitrites Cell Nucleus 030219 obstetrics & reproductive medicine Cell Death Chemistry NF-kappa B Trophoblast Biological Transport Th1 Cells In vitro Triterpenes Biomechanical Phenomena Trophoblasts 030104 developmental biology medicine.anatomical_structure Cytokines Female medicine.symptom |
| Zdroj: | Biochimica et biophysica acta. General subjects. 1863(9) |
| ISSN: | 1872-8006 |
| Popis: | Background Group B streptococcus (GBS) is the main bacteria that infects pregnant women and can cause abortion and chorioamnionitis. The impact of GBS effects on human trophoblast cells remains largely elusive, and actions toward anti-inflammatory strategies in pregnancy are needed. A potent anti-inflammatory molecule, uvaol is a triterpene from olive oil and its functions in trophoblasts are unknown. We aimed to analyze biomechanical and functional effects of inactivated GBS in trophoblast cells, with the addition of uvaol to test potential benefits. Methods HTR-8/SVneo cells were treated with uvaol and incubated with inactivated GBS. Cell viability and death were analyzed. Cellular elasticity and topography were accessed by atomic force microscopy. Nitrite production was evaluated by Griess reaction. Nuclear translocation of NFkB p65 was detected by immunofluorescence and Th1/Th2 cytokines by bead-based multiplex assay. Results GBS at 10 8 CFU increased cell death, which was partially prevented by uvaol. Cell stiffness, cytoskeleton organization and morphology were changed by GBS, and uvaol partially restored these alterations. Nuclear translocation of NFkB p65 began 15 min after GBS incubation and uvaol inhibited this process. GBS decreased IL-4 secretion and increased IL-1β, IFN-γ and IL-2, whereas uvaol reverted this. Conclusions The increased inflammation and cell death caused by GBS correlated with biomechanical and cytoskeleton changes found in trophoblast cells, while uvaol was effective its protective role. General significance Uvaol is a natural anti-inflammatory product efficient against GBS-induced inflammation and it has potential to be acquired through diet in order to prevent GBS deleterious effects in pregnancy. |
| Databáze: | OpenAIRE |
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