Molecular identification, tissue distribution and functional analysis of somatostatin receptors (SSTRs) in red-spotted grouper (Epinephelus akaara)
| Autor: | Wensheng Li, Weiru Lin, Zimu Tang, Caiyun Sun, Xi Yuan, Jirong Jia, Yazhou Zhang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
MAP Kinase Signaling System
030209 endocrinology & metabolism 03 medical and health sciences 0302 clinical medicine Endocrinology Sequence Analysis Protein Animals Humans Somatostatin receptor 2 Tissue Distribution Grouper Somatostatin receptor 1 Amino Acid Sequence RNA Messenger Receptors Somatostatin Receptor Peptide sequence Phylogeny 030304 developmental biology 0303 health sciences biology Somatostatin receptor Reproducibility of Results biology.organism_classification Cell biology Open reading frame HEK293 Cells Gene Expression Regulation Bass Female Animal Science and Zoology Signal transduction Somatostatin Proto-Oncogene Proteins c-akt |
| Zdroj: | General and Comparative Endocrinology. 274:87-96 |
| ISSN: | 0016-6480 |
| Popis: | In the present study, four full-length cDNAs of somatostatin receptor (sstr) were cloned from the forebrain and pituitary of red-spotted grouper. The four full-length cDNAs were designated 2292, 1522, 1873 and 1789 bp and identified as sstr1, sstr2, sstr3, and sstr5 by BLAST analysis; the corresponding sizes of the open reading frames (ORFs) were 1155, 1113, 1467 and 1503 bp, which encoding 384, 370, 488 and 500 aa, respectively. The four receptors have seven transmembrane structures and contain the YANSCANPI/VLY sequence, which is the conserved amino acid sequence of the SSTR family. A tissue distribution study showed that the four sstrs had different expression patterns, suggesting that they may play different roles in regulating different physiological processes. The four receptors mediate ERK1/2 phosphorylation by SS-14 in HEK293 cells, and SS-14 promotes ATK and ERK1/2 phosphorylation in primary hepatocytes of red-spotted grouper. These results facilitate the study of SSTRs-mediated intracellular signaling pathways. |
| Databáze: | OpenAIRE |
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