Development of a bienzymatic amperometric glucose biosensor using mesoporous silica (mcm-41) for enzyme immobilization and its application on liquid pharmaceutical formulations
| Autor: | Mario Aranda-Bustos, Jorge Carpinelli-Pavisic, Carlos Peña-Farfal, Claudio Zaror-Zaror, Noemi Caro-Jara, Rodolfo Mundaca-Uribe |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | ELECTROANALYSIS Artículos CONICYT CONICYT Chile instacron:CONICYT |
| Popis: | This paper describes the development and optimization of an amperometric bienzymatic biosensor for glucose, based on glucose oxidase (GOX) and peroxidase (HRP), immobilized on mesoporous silica with hexagonal symmetry (MCM-41). The mesoporous material was immobilized by Nafion assisted adsorption on a glassy carbon electrode, which was used as working electrode. Measurements were performed in a conventional three electrode cell using a Ag/AgCl reference electrode and platinum wire as counter electrode. Operating parameters such as GOX/HRP enzyme ratio, mediator (catechol) concentration, applied potential and pH were optimized. The proposed biosensor showed excellent analytical performance with detection and quantification limits of 8.6×10−6 M and 2.5×10−5 M, respectively; analytical sensitivity of 4.67×10−4 AM−1, repeatability |
| Databáze: | OpenAIRE |
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