Characterization of the primary spinal afferent innervation of the mouse colon using retrograde labelling
| Autor: | Gareth A. Hicks, D. R. Robinson, Peter A. McNaughton, M. L. Evans |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Pathology medicine.medical_specialty Colon Physiology Calcitonin Gene-Related Peptide Receptors Drug Population Calcitonin gene-related peptide Descending colon Mice Dorsal root ganglion Ganglia Spinal Lectins medicine Animals Neurons Afferent Receptor education Glycoproteins Afferent Pathways Mice Inbred BALB C education.field_of_study Staining and Labeling Receptors Purinergic P2 Endocrine and Autonomic Systems business.industry Gastroenterology Nociceptors Visceral pain Anatomy Immunohistochemistry medicine.anatomical_structure nervous system Calcitonin Nociceptor medicine.symptom business Receptors Purinergic P2X3 |
| Zdroj: | Neurogastroenterology and Motility. 16:113-124 |
| ISSN: | 1365-2982 1350-1925 |
| DOI: | 10.1046/j.1365-2982.2003.00456.x |
| Popis: | Visceral pain is the most common form of pain produced by disease and is thus of interest in the study of gastrointestinal (GI) complaints such as irritable bowel syndrome, in which sensory signals perceived as GI pain travel in extrinsic afferent neurones with cell bodies in the dorsal root ganglia (DRG). The DRG from which the primary spinal afferent innervation of the mouse descending colon arises are not well defined. This study has combined retrograde labelling and immunohistochemistry to identify and characterize these neurones. Small to medium-sized retrogradely labelled cell bodies were found in the DRG at levels T8-L1 and L6-S1. Calcitonin gene-related peptide (CGRP)- and P2X3-like immunoreactivity (LI) was seen in 81 and 32%, respectively, of retrogradely labelled cells, and 20% bound the Griffonia simplicifolia-derived isolectin IB4. CGRP-LI and IB4 were co-localized in 22% of retrogradely labelled cells, whilst P2X3-LI and IB4 were co-localized in 7% (vs 34% seen in the whole DRG population). Eighty-two per cent of retrogradely labelled cells exhibited vanilloid receptor 1-like immunoreactivity (VR1-LI). These data suggest that mouse colonic spinal primary afferent neurones are mostly peptidergic CGRP-containing, VR1-LI, C fibre afferents. In contrast to the general DRG population, a subset of neurones exist that are P2X3 receptor-LI but do not bind IB4. |
| Databáze: | OpenAIRE |
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