Biomolecular signatures of diabetic wound healing by structural mass spectrometry
| Autor: | John A. McLean, Jeffrey M. Davidson, Samir Ashfaq, John P. Wikswo, Susan R. Opalenik, Kelly M. Hines |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Diabetic rat Cholic Acid Mass spectrometry Article Analytical Chemistry Diabetes Mellitus Experimental Diabetes Complications Rats Sprague-Dawley chemistry.chemical_compound Diabetes mellitus medicine Animals Calgranulin A Wound Healing Chromatography integumentary system Chemistry Cholic acid Granulation tissue Lysophosphatidylcholines Equipment Design medicine.disease Rats medicine.anatomical_structure Polyvinyl Alcohol Diabetic wound healing Enzyme immunoassays Wound healing |
| Popis: | Wound fluid is a complex biological sample containing byproducts associated with the wound repair process. Contemporary techniques, such as immunoblotting and enzyme immunoassays, require extensive sample manipulation and do not permit the simultaneous analysis of multiple classes of biomolecular species. Structural mass spectrometry, implemented as ion mobility-mass spectrometry (IM-MS), comprises two sequential, gas-phase dispersion techniques well suited for the study of complex biological samples due to its ability to separate and simultaneously analyze multiple classes of biomolecules. As a model of diabetic wound healing, polyvinyl alcohol (PVA) sponges were inserted subcutaneously into non-diabetic (control) and streptozotocin-induced diabetic rats to elicit a granulation tissue response and to collect acute wound fluid. Sponges were harvested at days 2 or 5 to capture different stages of the early wound healing process. Utilizing IM-MS, statistical analysis, and targeted ultra-performance liquid chromatography (UPLC) analysis, biomolecular signatures of diabetic wound healing have been identified. The protein S100-A8 was highly enriched in the wound fluids collected from day 2 diabetic rats. Lysophosphatidylcholine (20:4) and cholic acid also contributed significantly to the differences between diabetic and control groups. This report provides a generalized workflow for wound fluid analysis demonstrated with a diabetic rat model. |
| Databáze: | OpenAIRE |
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