Combined modelling of mRNA decay dynamics and single-molecule imaging in the Drosophila embryo uncovers a role for P-bodies in 5′ to 3′ degradation
| Autor: | Lauren Forbes Beadle, Jennifer C. Love, Yuliya Shapovalova, Artem Artemev, Magnus Rattray, Hilary L. Ashe |
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| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Plos Biology, 21, 1, pp. 1-29 Plos Biology, 21, 1-29 Forbes-Beadle, L, Love, J, Shapovalova, Y, Artemev, A, Rattray, M & Ashe, H 2023, ' Combined modelling of mRNA decay dynamics and single-molecule imaging in the Drosophila embryo uncovers a role for P-bodies in 5′ to 3′ degradation ', PLoS Biology, vol. 21, no. 1, e3001956 . https://doi.org/10.1371/journal.pbio.3001956 |
| ISSN: | 1544-9173 |
| DOI: | 10.1371/journal.pbio.3001956 |
| Popis: | Regulation of mRNA degradation is critical for a diverse array of cellular processes and developmental cell fate decisions. Many methods for determining mRNA half-lives rely on transcriptional inhibition or metabolic labelling. Here, we use a non-invasive method for estimating half-lives for hundreds of mRNAs in the early Drosophila embryo. This approach uses the intronic and exonic reads from a total RNA-seq time series and Gaussian process regression to model the dynamics of premature and mature mRNAs. We show how regulation of mRNA stability is used to establish a range of mature mRNA dynamics during embryogenesis, despite shared transcription profiles. Using single-molecule imaging, we provide evidence that, for the mRNAs tested, there is a correlation between short half-life and mRNA association with P-bodies. Moreover, we detect an enrichment of mRNA 3′ ends in P-bodies in the early embryo, consistent with 5′ to 3′ degradation occurring in P-bodies for at least a subset of mRNAs. We discuss our findings in relation to recently published data suggesting that the primary function of P-bodies in other biological contexts is mRNA storage. |
| Databáze: | OpenAIRE |
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