Pantothenate auxotrophy in Zymomonas mobilis ZM4 is due to a lack of aspartate decarboxylase activity
| Autor: | Jennifer R Gliessman, James B. McKinlay, Timothy A. Kremer, Amee A Sangani, Sara E Jones-Burrage |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Carboxy-Lyases Auxotrophy Genetic Vectors 030106 microbiology Gene Expression medicine.disease_cause Microbiology Zymomonas mobilis Pantothenic Acid 03 medical and health sciences chemistry.chemical_compound Pantothenic acid Genetics medicine Molecular Biology Escherichia coli Amino acid synthesis chemistry.chemical_classification Zymomonas Growth medium Ethanol biology Chemistry Escherichia coli Proteins biology.organism_classification Culture Media 030104 developmental biology Biochemistry Fermentation beta-Alanine Heterologous expression Amino Acids Branched-Chain |
| Zdroj: | FEMS Microbiology Letters. 364 |
| ISSN: | 1574-6968 |
| DOI: | 10.1093/femsle/fnx136 |
| Popis: | The bacterium Zymomonas mobilis naturally produces ethanol at near theoretical maximum yields, making it of interest for industrial ethanol production. Zymomonas mobilis requires the vitamin pantothenate for growth. Here we characterized the genetic basis for the Z. mobilis pantothenate auxotrophy. We found that this auxotrophy is due to the absence of a single gene, panD, encoding aspartate-decarboxylase. Heterologous expression of Escherichia coli PanD in Z. mobilis or supplementation of the growth medium with the product of PanD activity, β-alanine, eliminated the need for exogenous pantothenate. We also determined that Z. mobilis IlvC, an enzyme better known for branched-chain amino acid synthesis, is required for pantothenate synthesis in Z. mobilis, as it compensates for the absence of PanE, another pantothenate synthesis pathway enzyme. In addition to contributing to an understanding of the nutritional requirements of Z. mobilis, our results have led to the design of a more cost-effective growth medium. |
| Databáze: | OpenAIRE |
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