Effect of intravenous immunoglobulin G on natural killer cell cytotoxicity in vitro in women with recurrent spontaneous abortion
| Autor: | Joanne Y.H. Kwak, Yoon B. Kim, Linda L. Baum, Jaime E. Ruiz, Kenneth D. Beaman, Alan E. Beer, Alice Gilman-Sachs |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Cytotoxicity
Immunologic Abortion Habitual Immunology chemical and pharmacologic phenomena Biology Immunoglobulin G Natural killer cell Flow cytometry Immunoglobulin Fab Fragments Pregnancy hemic and lymphatic diseases Tumor Cells Cultured medicine Humans Immunology and Allergy Cytotoxicity Antibody-dependent cell-mediated cytotoxicity medicine.diagnostic_test Antibody-Dependent Cell Cytotoxicity Immunoglobulins Intravenous Obstetrics and Gynecology medicine.disease In vitro Killer Cells Natural Leukemia medicine.anatomical_structure Reproductive Medicine Leukemia Myeloid biology.protein Female Antibody |
| Zdroj: | Journal of Reproductive Immunology. 31:125-141 |
| ISSN: | 0165-0378 |
| DOI: | 10.1016/0165-0378(96)00969-2 |
| Popis: | Intravenous immunoglobulin (IVIg) has been used to treat women with recurrent spontaneous abortion (RSA), particularly for women with elevated natural killer (NK) cells. We investigated the effect of IVIg on peripheral blood NK cell activity in vitro in women with RSA. 51Cr-release assays using K562 in the presence of varying concentrations of IVIg were performed using PBL from 16 women with RSA. Antibody dependent cellular cytotoxicity (ADCC) was evaluated using Daudi cells. Effectors and targets were preincubated with IVIg. Binding of IVIg to K562 and Daudi was evaluated by flow cytometry. The effect of K562 absorbed IVIg on NK activity was compared to that of non-absorbed IVIg. NK cytotoxicity and ADCC in the presence of F(ab')2 fragments were compared with those in the presence of intact IVIg. IVIg produced a significant, dose dependent inhibition of NK activity in vitro. Inhibition of NK activity occurred when effectors but not targets were preincubated with IVIg. IVIg binds to K562 and Daudi. IVIg increased ADCC when targets but not effectors were incubated with IVIg. K562 absorbed IVIg produced more inhibition of NK cytotoxicity than non-absorbed IVIg. Suppression of NK cytotoxicity by F(ab')2 was as effective as that of IVIg. However, F(ab')2 did not increase ADCC. IVIg effectively reduces peripheral blood NK cytotoxicity in vitro. Inhibition of NK cytotoxicity is mediated at the effector cell level through the antigen binding portion of the immunoglobulins. Women with RSA and elevated NK cells may benefit from IVIg treatment. |
| Databáze: | OpenAIRE |
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