Characterisation of Mycoplasma capricolum P60 surface lipoprotein and its evaluation in a recombinant ELISA
| Autor: | Ennio Bandino, Tiziana Cubeddu, Richard Thiéry, Pascale Mercier, Sergio Rosati, Alberto Alberti, Marco Pittau, Margherita Profiti, Maria Filippa Addis, Patrizia Robino, Bernardo Chessa, Alessandro Mannelli |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Basic membrane proteins
P60 Lipoproteins Recombinant Fusion Proteins Mycoplasma agalactiae Molecular Sequence Data ved/biology.organism_classification_rank.species Enzyme-Linked Immunosorbent Assay Mycoplasmataceae Sensitivity and Specificity Microbiology Mycoplasma capricolum law.invention Bacterial Proteins Antigen law Animals Point Mutation Mycoplasma capricolum subspecies capricolum Cloning Molecular Pleuropneumonia Contagious Contagious agalactia Goat Diseases Base Sequence General Veterinary biology ved/biology Goats food and beverages General Medicine ELISA biology.organism_classification Antibodies Bacterial Membrane protein Recombinant DNA Mollicutes Rabbits Bacteria |
| Zdroj: | Veterinary Microbiology. 128:81-89 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2007.09.020 |
| Popis: | This paper reports the identification and characterisation of a 60 kDa surface protein antigen (P60) of Mycoplasma capricolum subspecies capricolum (Mcc), and describes its diagnostic application. Genomic localization and presence in P60 of conserved functional domains suggested a structural and functional relationship with the immunodominant antigen P48 of Mycoplasma agalactiae, a basic membrane protein. A rP60-ELISA was developed, and it resulted in a high specificity for Mcc infections after evaluation with 125 goat sera. The comparison with an existent ELISA based on whole Mcc cell lysates showed that the two assays have comparable sensitivities, but the rP60-ELISA has the significant advantage of a greater specificity. Results indicate that P60 is a potential marker of Mcc infection, especially useful in areas where the presence of M. capricolum subspecies capripenumoniae is also reported. |
| Databáze: | OpenAIRE |
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