Characteristics of Circular RNA Expression Profiles of Porcine Granulosa Cells in Healthy and Atretic Antral Follicles

Autor: Chi Chiu Wang, Jian Tao, Zhihong Zhao, Marcel Jaklofsky, Hengxi Wei, Shouquan Zhang, Yaodi Liang, Li Li, Li Meng, Katja J. Teerds
Rok vydání: 2020
Předmět:
antral follicular atresia
0301 basic medicine
endocrine system
Swine
Follicular Atresia
CircRNA expression profiles
Apoptosis
MiRNA binding
Biology
Article
Catalysis
lcsh:Chemistry
Inorganic Chemistry
03 medical and health sciences
Circular RNA
Animals
Granulosa cell apoptosis
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Gene
Spectroscopy
VLAG
Messenger RNA
Granulosa Cells
030102 biochemistry & molecular biology
Follicular atresia
Organic Chemistry
granulosa cell apoptosis
RNA
Circular
General Medicine
Antral follicle
Molecular biology
Fold change
Computer Science Applications
030104 developmental biology
Gene Expression Regulation
lcsh:Biology (General)
lcsh:QD1-999
Human and Animal Physiology
Antral follicular atresia
circRNA expression profiles
WIAS
Fysiologie van Mens en Dier
Female
Transcriptome
Immunostaining
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 15
International Journal of Molecular Sciences, Vol 21, Iss 5217, p 5217 (2020)
International Journal of Molecular Sciences, 21(15)
International Journal of Molecular Sciences 21 (2020) 15
ISSN: 1422-0067
DOI: 10.3390/ijms21155217
Popis: Circular RNAs (circRNAs) are thought to play essential roles in multiple biological processes, including apoptosis, an important process in antral follicle atresia. We aimed to investigate the potential involvement of circRNAs in granulosa cell apoptosis and thus antral follicle atresia. CircRNA expression profiles were generated from porcine granulosa cells isolated from healthy antral (HA) and atretic antral (AA) follicles. Over 9632 circRNAs were identified, of which 62 circRNAs were differentially expressed (DE-circRNAs). Back-splicing, RNase R resistance, and stability of DE-circRNAs were validated, and miRNA binding sites and related target genes were predicted. Two exonic circRNAs with low false discovery rate (FDR) high fold change, miRNA binding sites, and relevant biological functions&mdash
circ_CBFA2T2 and circ_KIF16B&mdash
were selected for further characterization. qRT-PCR and linear regression analysis confirmed expression and correlation of the targeted genes&mdash
the antioxidant gene GCLC (potential target of circ_CBFA2T2) and the apoptotic gene TP53 (potential target of circ_KIF16B). Increased mRNA content of TP53 in granulosa cells of AA follicles was further confirmed by strong immunostaining of both p53 and its downstream target pleckstrin homology like domain family a member 3 (PHLDA3) in AA follicles compared to negligible staining in granulosa cells of HA follicles. Therefore, we concluded that aberrantly expressed circRNAs presumably play a potential role in antral follicular atresia.
Databáze: OpenAIRE
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