Inhibition of N-myc downstream-regulated gene 2 in prostatic carcinoma
| Autor: | Wei Yan, Libo Yao, Wei Zhang, Lei Gao, Nana Dang, Noor Beckwith, Jianlin Yuan, Rui Zhang, Guojun Wu, Chuigong Yu, Yingmei Wang, Yi Zhao |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
Cancer Research Blotting Western Transplantation Heterologous Prostatic Hyperplasia Mice Nude Biology urologic and male genital diseases medicine.disease_cause Adenoviridae Flow cytometry Mice Nude mouse Microscopy Electron Transmission Transduction Genetic In vivo Cell Line Tumor medicine Animals Humans Aged Cell Proliferation Retrospective Studies Aged 80 and over Pharmacology Regulation of gene expression Mice Inbred BALB C medicine.diagnostic_test Cell growth Tumor Suppressor Proteins Prostate Prostatic Neoplasms Middle Aged Hyperplasia biology.organism_classification medicine.disease Immunohistochemistry Gene Expression Regulation Neoplastic Oncology Cell culture Cancer research Molecular Medicine Neoplasm Grading Carcinogenesis Neoplasm Transplantation |
| Zdroj: | Cancer Biology & Therapy. 12:304-313 |
| ISSN: | 1555-8576 1538-4047 |
| DOI: | 10.4161/cbt.12.4.16382 |
| Popis: | To study the expression of N-myc Downstream Regulated Gene-2 (NDRG2) in prostatic carcinoma (PCA) tissue and in different PCA cell lines, and to investigate its clinical and pathological implications, 144 PCA and benign prostatic hyperplasia (BPH) tissue sections were analyzed retrospectively with immunohistochemistry (S-P method). The expression levels of NDRG2 and c-Myc in prostate cell lines were detected through Western blot. The effects of adenovirus-mediated NDRG2 on PC3 cells and PC3 nude mouse xenografts was observed through cell growth curves, tumor growth curves, flow cytometry (FCM), transmission electron microscopy (TEM) and TUNEL staining. The NDRG2 gene was highly expressed in BPH tissues, but not in carcinomatous ones (χ(2)=25.98, p0.001). Furthermore, positive expression of NDRG2 was negatively correlated with the Gleason score (r = -0.445, p0.001) and the c-myc level (r = -0.311, p0.001). However, positive expression of NDRG2 was not correlated with pTNM tumor stages or the serum concentration of prostate-specific antigen (PSA) (p0.05). The expression of the NDRG2 genes was low in the three PCA cell lines. PC3 cells infected by pAD-cmv-NDRG2 showed inhibition of proliferation both in vitro and vivo. To sum up, NDRG2 may be involved in the carcinogenesis and progression of PCA. Moreover, adenovirus-mediated NDRG2 can suppress the proliferation of PC3 cells significantly both in vitro and in vivo. These results indicate that NDRG2 may become a new target gene for PCA diagnosis and therapy. |
| Databáze: | OpenAIRE |
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