cDNA microarray analysis reveals novel candidate genes expressed in human peripheral blood following exhaustive exercise
| Autor: | Kay Nieselt, Elvira Fehrenbach, Judith Fliegner, Andreas M. Niess, Rainer Spanagel, Derek Zieker, Peter J. Gebicke-Haerter, Marc Waidmann, Perikles Simon, Janko Dietzsch, Hinnak Northoff |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Candidate gene DNA Complementary Physiology Sialoglycoproteins Computational biology Protein Serine-Threonine Kinases Biology Bioinformatics Running Tetraspanin 28 Immune system Antigens CD Endurance training Complementary DNA Genetics Humans Exercise DNA Primers Glutathione Transferase Oligonucleotide Array Sequence Analysis Microarray analysis techniques Intracellular Signaling Peptides and Proteins Blood Proteins Peripheral Interleukin 1 Receptor Antagonist Protein Gene Expression Regulation RNA Thioredoxin CD81 |
| Zdroj: | Physiological Genomics. 23:287-294 |
| ISSN: | 1531-2267 1094-8341 |
| DOI: | 10.1152/physiolgenomics.00096.2005 |
| Popis: | It is generally accepted that exhausting endurance exercise exhibits strong effects on the immune system. Such effects have been attributed to changes in the cellular composition of peripheral blood as well as to changes in the expression of plausible candidate genes. The list of candidate genes is far from being complete, since this issue has not yet been investigated in a systematic way. In this study, we used a custom-made cDNA microarray focused on inflammation as a screening approach to study gene expression in eight one-half marathon runners before, immediately after, and 24 h after exercise. Significant differential gene expression was verified by quantitative real-time PCR. Linear regression analysis showed that microarray expression analysis of cell type-specific surface molecules reflects the observed individual cellular shifts in peripheral blood cells with high statistical significance. In line with the results of former studies, we observed an upregulation of mitogen-activated protein kinase-activated protein kinase-2 (MAPKAP-K2), l-selectin, and IL-1 receptor antagonist (IL-1ra) after exhaustive exercise. The main results of this study report, for the first time, the downregulation of CD81; the upregulation of thioredoxin, which may play an important part in anti-oxidative defense; and, surprisingly, the downregulation of the anti-carcinogenic gene glutathione- S-transferase-3 (GSTM3) in peripheral blood. The study shows cDNA microarray expression analysis as a reliable systematic instrument to complete the list of candidate genes that may play a role in exhaustive exercise-induced modulation of the immune response. |
| Databáze: | OpenAIRE |
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