Additional file 1 of Identification of Y chromosome markers in the eastern three-lined skink (Bassiana duperreyi) using in silico whole genome subtraction

Autor: Dissanayake, Duminda Sampath Bandara, Holleley, Clare Ellen, Hill, Laura Kate, O’Meally, Denis, Deakin, Janine Eileen, Georges, Arthur
Rok vydání: 2020
DOI: 10.6084/m9.figshare.13024095.v1
Popis: Additional file 1: Figure S1. K-mer spectrum for the genome sequence of a male B. duperreyi. Figure S2. K-mer spectrum for the genome sequence of a female B. duperreyi. Figure S3. Number of Y enriched contigs ranging from 80 bp to 1374 bp resulting from the inchworm assembler. Figure S4. Contig length (bp) for the 92 subtraction contigs selected for PCR-based screening. Figure S5. Sequencing coverage for the 92 subtraction contigs selected for PCR-based screening. Figure S6. External and histological views of a) ovary b) testis in adult individuals of B. duperreyi. Figure S7. Karyotype of a male scincid lizard Bassiana duperreyi. Figure S8. Sequence alignment (a) and phylogeny (b) of bdM27_23_X5_798 contigs (top blue color highlight) with amplified 4 males (Piccadilly Circus_ACT) and 4 males (Anglesea _VIC). Figure S9. Sequence alignment (a) and phylogeny (b) of bdM27_10_X7_874 contigs (top blue color highlight) with amplified 4 males (Piccadilly Circus_ACT) and 4 males (Anglesea _VIC). Figure S10. Sequence alignment of bdM27_74_X11_649 contigs (top blue color highlight) with amplified 4 males (Piccadilly Circus_ACT) and 4 males (Anglesea _VIC). Figure S11. Sequence alignment (a) and phylogeny (b) of bdM27_82_X5_636 contigs (top blue color highlight) with amplified 2 males (Piccadilly Circus_ACT) and 4 males (Anglesea _VIC). Figure S12. Sequence alignment (a) and phylogeny (b) of bdM27_79_X5_643 contigs (top blue color highlight) with amplified 4 males (Piccadilly Circus_ACT) and 4 males (Anglesea _VIC). Figure S13. Sequence alignment (a) and phylogeny of bdM27_69_X9_658 contigs (top blue color highlight) with amplified 4 males (Piccadilly Circus_ACT) and 4 males (Anglesea _VIC). Figure S14. Sequence alignment of bdM27_87_X6_628 contigs (top blue color highlight) with amplified 4 males (Piccadilly Circus_ACT) and 4 males (Anglesea _VIC). Table S1. Estimates of evolutionary divergence between Piccadilly Circus and Anglesea individuals of Bassiana duperryi. Table S2. BLAST results for Y-specific contigs queried against representative reptile genomes. Table S3. Hits to known repeats in the Dfam database. Table S4. Specimen data, sex, locality and measurements for the Bassiana duperreyi specimens used in this study.
Databáze: OpenAIRE