Hypoxia and inactivity related physiological changes precede or take place in absence of significant rearrangements in bacterial community structure: The PlanHab randomized trial pilot study

Autor: Zala Prevoršek, Jenna Chandler, Igor B. Mekjavic, Michael Schloter, Robert Šket, James M. Tiedje, Nicole Simone Treichel, Matevž Likar, Ola Eiken, Marius Vital, Tadej Debevec, Susanne Kublik, Blaž Stres, Boštjan Murovec
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
medicine.medical_treatment
lcsh:Medicine
Pilot Projects
Bed rest
Database and Informatics Methods
Feces
RNA
Ribosomal
16S
Medicine and Health Sciences
Bacteroides
Hypoxia
lcsh:Science
Chromatography
High Pressure Liquid
Cross-Over Studies
Multidisciplinary
Ecology
biology
Chemistry
Genomics
Healthy Volunteers
Community Ecology
Medical Microbiology
Anatomy
medicine.symptom
Sequence Analysis
Research Article
medicine.medical_specialty
Bioinformatics
030106 microbiology
Sequence Databases
Microbial Genomics
Research and Analysis Methods
Bacterial Physiological Phenomena
Microbiology
03 medical and health sciences
Internal medicine
Genetics
medicine
Humans
Circadian rhythm
Community Structure
Exercise
Nutrition
Bacteria
Gut Bacteria
Ecology and Environmental Sciences
lcsh:R
Organisms
Biology and Life Sciences
Cell Biology
Hypoxia (medical)
biology.organism_classification
Crossover study
Sterol
Diet
Gastrointestinal Tract
Biological Databases
030104 developmental biology
Endocrinology
Defecation
lcsh:Q
Microbiome
Digestive System
Zdroj: PLoS ONE, Vol 12, Iss 12, p e0188556 (2017)
PLoS ONE
PLoS ONE 12:e0188556 (2017)
ISSN: 1932-6203
Popis: We explored the assembly of intestinal microbiota in healthy male participants during the randomized crossover design of run-in (5 day) and experimental phases (21-day normoxic bed rest (NBR), hypoxic bed rest (HBR) and hypoxic ambulation (HAmb) in a strictly controlled laboratory environment, with balanced fluid and dietary intakes, controlled circadian rhythm, microbial ambiental burden and 24/7 medical surveillance. The fraction of inspired O2 (FiO2) and partial pressure of inspired O2 (PiO2) were 0.209 and 133.1 ± 0.3 mmHg for NBR and 0.141 ± 0.004 and 90.0 ± 0.4 mmHg for both hypoxic variants (HBR and HAmb; ~4000 m simulated altitude), respectively. A number of parameters linked to intestinal environment such as defecation frequency, intestinal electrical conductivity (IEC), sterol and polyphenol content and diversity, indole, aromaticity and spectral characteristics of dissolved organic matter (DOM) were measured (64 variables). The structure and diversity of bacterial microbial community was assessed using 16S rRNA amplicon sequencing. Inactivity negatively affected frequency of defecation and in combination with hypoxia increased IEC (p < 0.05). In contrast, sterol and polyphenol diversity and content, various characteristics of DOM and aromatic compounds, the structure and diversity of bacterial microbial community were not significantly affected over time. A new in-house PlanHab database was established to integrate all measured variables on host physiology, diet, experiment, immune and metabolic markers (n = 231). The observed progressive decrease in defecation frequency and concomitant increase in IEC suggested that the transition from healthy physiological state towards the developed symptoms of low magnitude obesity-related syndromes was dose dependent on the extent of time spent in inactivity and preceded or took place in absence of significant rearrangements in bacterial microbial community. Species B. thetaiotamicron, B. fragilis, B. dorei and other Bacteroides with reported relevance for dysbiotic medical conditions were significantly enriched in HBR, characterized with most severe inflammation symptoms, indicating a shift towards host mucin degradation and proinflammatory immune crosstalk.
Databáze: OpenAIRE
Externí odkaz: