Inhibitory effect of a long-acting somatostatin analogue on EGF-stimulated cell proliferation in Capan-2 cells
| Autor: | Thomas M. Gress, Beáta Burghardt, Zoltán Marcsek, Flautner L, Gábor Varga, Kornélia Barabás |
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| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_specialty
Biology Adenocarcinoma Octreotide Epidermal growth factor Physiology (medical) Internal medicine medicine Tumor Cells Cultured Somatostatin receptor 2 Humans Protein Isoforms Receptors Somatostatin Receptor Cholecystokinin Delta cell Epidermal Growth Factor Cell growth General Neuroscience DNA Receptor Cholecystokinin B Receptor Cholecystokinin A Pancreatic Neoplasms medicine.anatomical_structure Endocrinology Somatostatin Receptors Cholecystokinin Pancreas hormones hormone substitutes and hormone antagonists Cell Division Thymidine |
| Zdroj: | Journal of physiology, Paris. 94(1) |
| ISSN: | 0928-4257 |
| Popis: | Numerous studies have reported diverse effects of gut-derived regulatory peptides on growth of the normal pancreas, pancreatic neoplasms induced experimentally in animals, and pancreatic cancer cell lines, but the results of these investigations are rather controversial. The stimulatory effect of epidermal growth factor (EGF) on cell proliferation of pancreatic cell lines is well established. Whether this action can be modulated by somatostatin is not clear. Furthermore, it is not certain whether another regulatory peptide, cholecystokinin (CCK), affects the proliferation of these cells. In the present study we investigated the presence of CCK-A and CCK-B, as well as somatostatin-2 (SSTR2) receptors by RT-PCR, and studied the actions of EGF, CCK and octreotide on DNA synthesis in the human pancreatic adenocarcinoma cell line Capan-2. Octreotide, a long-acting somatostatin analogue was used as somatostatin agonist. Cells were cultured in RPMI-1640 medium. They were incubated in serum free medium containing 0.2% BSA in the absence (control) or the presence of the peptides. [3H]-thymidine incorporation into DNA was measured after 48 h of incubation. By means of RT-PCR analysis we were able to demonstrate SSTR2 expression, but not CCK-A or CCK-B receptor mRNA in Capan-2 cells. DNA synthesis evaluated by [3H]-thymidine incorporation was found to be increased by 45.2 +/- 5.6% in response to EGF (10(-8) M) and decreased by 11.7 +/- 2.6% to octreotide (10(-8) M) compared to controls (P0.01). The increase in [3H]-thymidine incorporation was significantly lower when EGF treatment was combined with octreotide administration (10.1 +/- 2.5% over control). In the concentration range of 10(-11)-10(-8) M, CCK did not alter significantly the incorporation of [3H]-thymidine into DNA in Capan-2 cells. In conclusion, these data support a role for EGF as a growth factor for the human pancreatic cancer cell Capan-2. Somatostatin may play an important role in regulating cell proliferation in Capan-2 cells both under basal, and growth factor-stimulated conditions. Our results suggest, however, that CCK receptors are not expressed, and CCK does not affect cell proliferation in this transformed pancreatic cell line. |
| Databáze: | OpenAIRE |
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