Cyclic AMP Decreases the Expression of a Neuronal Marker (GAD67) and Increases the Expression of an Astroglial Marker (GFAP) in C6 Cells
| Autor: | Jose Segovia, George M. Lawless, Allan J. Tobin, Michael Brenner, Niranjala J.K. Tillakaratne |
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| Rok vydání: | 2002 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Glutamate decarboxylase Gene Expression Biology Polymerase Chain Reaction Biochemistry Isozyme Cell Line Cellular and Molecular Neuroscience Transcription (biology) Glial Fibrillary Acidic Protein Cyclic AMP Tumor Cells Cultured medicine Animals RNA Messenger RNA Neoplasm DNA Primers Neurons Messenger RNA Reporter gene Base Sequence Glial fibrillary acidic protein Glutamate Decarboxylase Colforsin Brain Glioma Molecular biology Actins Rats Kinetics medicine.anatomical_structure nervous system Astrocytes biology.protein Biomarkers Intracellular Astrocyte |
| Zdroj: | Journal of Neurochemistry. 63:1218-1225 |
| ISSN: | 0022-3042 |
| Popis: | C6 cells express proteins and mRNAs that are characteristic of both glia and neurons. Agents that increase intracellular levels of cyclic AMP (cAMP) decrease the enzymatic activity of glutamate decarboxylase (GAD), a neuronal marker, and the mRNA levels for one of the two GAD isoenzymes, GAD67. This reduction is accompanied by increased levels of glial fibrillary acidic protein (GFAP) mRNA, an astrocyte marker. Transient transfection assays, in which a 2-kb upstream regulatory region of the human GFAP gene was linked to a reporter gene, indicate that at least some of the cAMP-mediated increase of GFAP mRNA levels is due to increased transcription. Increases in intracellular cAMP appear to induce differentiation of C6 cells toward a more mature astrocyte phenotype. |
| Databáze: | OpenAIRE |
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