A new specific reference gene based on growth hormone gene (GH1) used for detection and relative quantification of Aquadvantage® GM salmon (Salmo salar L.) in food products
| Autor: | Ahmed Ben Hafsa, Khaled Said, Maher Chaouachi, Mohamed Salem Zellama, Nesrine Nabi |
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| Rok vydání: | 2014 |
| Předmět: |
Food
Genetically Modified Salmo salar Aquaculture Biology Real-Time Polymerase Chain Reaction 01 natural sciences Analytical Chemistry law.invention 0404 agricultural biotechnology law Animals Food science Salmo Polymerase chain reaction business.industry 010401 analytical chemistry AquAdvantage salmon 04 agricultural and veterinary sciences General Medicine biology.organism_classification 040401 food science 0104 chemical sciences Biotechnology Genetically modified organism genomic DNA Real-time polymerase chain reaction Food Growth Hormone Food processing business Food Science |
| Zdroj: | Food chemistry. 190 |
| ISSN: | 1873-7072 |
| Popis: | Genetic transformation of fish is mainly oriented towards the improvement of growth for the benefit of the aquaculture. Actually, Atlantic salmon (Salmo salar) is the species most transformed to achieve growth rates quite large compared to the wild. To anticipate the presence of contaminations with GM salmon in fish markets and the lack of labeling regulations with a mandatory threshold, the proper methods are needed to test the authenticity of the ingredients. A quantitative real-time polymerase chain reaction (QRT-PCR) method was used in this study. Ct values were obtained and validated using 15 processed food containing salmon. The relative and absolute limits of detection were 0.01% and 0.01 ng/μl of genomic DNA, respectively. Results demonstrate that the developed QRT-PCR method is suitable specifically for identification of S. salar in food ingredients based on the salmon growth hormone gene 1 (GH1). The processes used to develop the specific salmon reference gene case study are intended to serve as a model for performing quantification of Aquadvantage® GM salmon on future genetically modified (GM) fish to be commercialized. |
| Databáze: | OpenAIRE |
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