A novel intracellular nitrogen‐fixing symbiosis made by U stilago maydis and B acillus spp
| Autor: | José Ruiz-Herrera, Holjes Salgado-Lugo, Roberto Ruiz-Medrano, Antonio Vera-Nuñez, Claudia G. León-Ramírez, Alejandro Sánchez-Arreguín, Lino Sánchez-Segura, Juan José Peña-Cabriales |
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| Rok vydání: | 2015 |
| Předmět: |
Nitrogen
Physiology Ustilago Molecular Sequence Data Intracellular Space Bacillus Plant Science Microbiology Symbiosis Nitrogen Fixation RNA Ribosomal 16S Nitrogenase In Situ Hybridization Fluorescence Phylogeny Electrophoresis Agar Gel Nitrogen Isotopes biology Acetylene Bacillus pumilus Intracellular parasite Pathogenic fungus biology.organism_classification Anti-Bacterial Agents Nitrogen fixation Bacteria |
| Zdroj: | New Phytologist. 207:769-777 |
| ISSN: | 1469-8137 0028-646X |
| DOI: | 10.1111/nph.13359 |
| Popis: | We observed that the maize pathogenic fungus Ustilago maydis grew in nitrogen (N)-free media at a rate similar to that observed in media containing ammonium nitrate, suggesting that it was able to fix atmospheric N2 . Because only prokaryotic organisms have the capacity to reduce N2 , we entertained the possibility that U. maydis was associated with an intracellular bacterium. The presence of nitrogenase in the fungus was analyzed by acetylene reduction, and capacity to fix N2 by use of (15) N2 . Presence of an intracellular N2 -fixing bacterium was analyzed by PCR amplification of bacterial 16S rRNA and nifH genes, and by microscopic observations. Nitrogenase activity and (15) N incorporation into the cells proved that U. maydis fixed N2 . Light and electron microscopy, and fluorescence in situ hybridization (FISH) experiments revealed the presence of intracellular bacteria related to Bacillus pumilus, as evidenced by sequencing of the PCR-amplified fragments. These observations reveal for the first time the existence of an endosymbiotic N2 -fixing association involving a fungus and a bacterium. |
| Databáze: | OpenAIRE |
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