Solubilization of binary lipid mixtures by the detergent Triton X-100: the role of cholesterol
| Autor: | Bruno Mattei, Ana D. C. França, Karin A. Riske |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Chromatography
Octoxynol Bilayer Vesicle technology industry and agriculture Phospholipid Surfaces and Interfaces Condensed Matter Physics Lipids chemistry.chemical_compound Cholesterol chemistry Microscopy Fluorescence Solubility Phase (matter) Triton X-100 Electrochemistry lipids (amino acids peptides and proteins) General Materials Science Sphingomyelin Lipid bilayer POPC Spectroscopy |
| Zdroj: | Langmuir : the ACS journal of surfaces and colloids. 31(1) |
| ISSN: | 1520-5827 |
| Popis: | The solubilization of lipid bilayers of different composition and phase by the detergent Triton X-100 (Triton X-100) was investigated using optical and fluorescence microscopy of giant unilamellar vesicles (GUVs) and light scattering of large unilamellar vesicles (LUVs). The compositions explored were 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), in the liquid-disordered (Ld) phase, sphingomyelin (SM), in the gel phase, and binary mixtures of these phospholipids with 30 mol % cholesterol (chol), resulting in bilayers in the Ld and liquid-ordered (Lo) phases, respectively. We show that the phospholipid bilayers are completely soluble in TX-100, but optical microscopy reveals that whereas fluid POPC is gradually solubilized by TX-100, gel SM is first shattered in bilayer fragments. Incorporation of TX-100 in the membrane leads to increase in GUV area, which was quantified and expressed as bound detergent-to-lipid molar ratio. The partition of TX-100 in POPC is very high, decreases in POPC/chol, and is negligible in SM/chol. Fluorescence microscopy shows that TX-100 induces Lo/Ld phase separation in previously homogeneous POPC/chol GUVs, and insoluble bilayer fragments/vesicles are detected with optical microscopy and light scattering. Vesicles of SM/chol, in the Lo phase, are virtually insoluble in TX-100. Taken together, our results show that the presence of cholesterol is the origin of membrane resistance to solubilization, which depending on the specific lipid mixture can result in either partially (POPC/chol) or completely (SM/chol) insoluble mixtures. |
| Databáze: | OpenAIRE |
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