Immune Response in Calves Vaccinated with Type Three Secretion System Antigens and Shiga Toxin 2B Subunit of Escherichia coli O157:H7
| Autor: | Ángel Adrián Cataldi, Marina S. Palermo, Luisina Martorelli, Daniel A. Vilte, E.C. Mercado, Cristina Ibarra, Sergio Garbaccio, Maria Pilar Mejias, Adriana Andrea Albanese |
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| Rok vydání: | 2017 |
| Předmět: |
Bacterial Diseases
Male 0301 basic medicine Erythrocytes Respuesta Inmunológica Physiology lcsh:Medicine Calves Toxicology Pathology and Laboratory Medicine medicine.disease_cause Biochemistry Shiga Toxin 2 Antígenos Bacterianos Immune Physiology hemic and lymphatic diseases Chlorocebus aethiops Medicine and Health Sciences Type III Secretion Systems Toxins Public and Occupational Health Enzyme-Linked Immunoassays lcsh:Science Immune Response Escherichia coli Infections Mammals Bacterial Shedding Escherichia Coli Immune System Proteins Multidisciplinary Virulence biology Escherichia coli Vaccines Escherichia coli Proteins Vaccination Agriculture Shiga toxin Ruminants Vaccination and Immunization Antibodies Bacterial Recombinant Proteins Infectious Diseases Toxina Shiga Vertebrates Antibody Research Article Bacterial Outer Membrane Proteins Livestock Bacterial Antigens Immunology Toxic Agents 030106 microbiology Cattle Diseases Research and Analysis Methods Escherichia coli O157 Antibodies Microbiology 03 medical and health sciences Immune system Antigen Bovines Cell Adhesion medicine Animals Antigens Immunoassays Adhesins Bacterial Vero Cells Escherichia coli Intimin lcsh:R Vacunación Organisms Biology and Life Sciences Proteins Virology Immunity Humoral Bacterial adhesin 030104 developmental biology Immunoglobulin G Amniotes Immunologic Techniques biology.protein lcsh:Q Cattle Preventive Medicine Ternero |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 1, p e0169422 (2017) PLoS One 12(1) : 2017 INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0169422 |
| Popis: | Ruminants are the primary reservoir of Shiga-toxin producing Escherichia coli (STEC) O157:H7 and the main source of infection for humans. The aim of this study was to assess the immunogenic properties of a candidate vaccine consisting on the recombinant proteins of E. coli O157:H7 IntiminC280, the carboxy-terminal fraction of Intimin γ, EspB and the fusion protein between the B subunit of Stx2 and Brucella Lumazine Synthase (BLS)(BLS-Stx2B), in Holstein Fresian calves.To accomplish this goal we vaccinated calves with two doses of different vaccine formulations: 2 antigens (IntiminC280, EspB), 3 antigens (IntiminC280, EspB, BLS-Stx2B), BLS-Stx2B alone and a control non-vaccinated group. All antigens were expressed as recombinant proteins in E. coli. Specific IgG titres increased in vaccinated calves and the inclusion of BLS-Stx2B in the formulation seems to have a stimulatory effect on the humoral response to IntiminC280 and EspB after the booster. The neutralizing activity of antibodies against these two antigens was assessed in Red Blood Cell lysis assays and adherence to Hep-2 cells as a correlate of T3SS activity. Both sera from animals vaccinated with 2 or 3 antigens inhibited both virulence properties. Serological response to Stx2 was observed in animals vaccinated only with BLS-Stx2B and with 3 antigens and neutralization of Stx2 cytotoxicity was also observed in both groups. In conclusion, immunization of calves with BLS-Stx2B, IntiminC280 and EspB elicited a potent humoral response able to neutralize Shiga toxin 2 cytotoxity and the T3SS virulence properties in vitro. These results suggest that this formulation is a good candidate vaccine to reduce STEC shedding in cattle and needs to be further assessed in vivo Inst. de Patobiología Fil: Martorelli, Luisina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Albanese, Adriana Andrea. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Mejias, María Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos; Argentina Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina. Academia Nacional de Medicina de Buenos Aires. Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos; Argentina Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Ibarra, Cristina E. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina |
| Databáze: | OpenAIRE |
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