Development and Comparison of Enzyme Immunoassays for Diagnosis of Chagas' Disease Using Fixed Forms of Trypanosoma cruzi (Epimastigotes, Amastigotes, and Trypomastigotes) and Assessment of Antigen Stability for the Three Assays
| Autor: | Brian J. Ward, Sonia Lacouture, Alberto Aché, Fabio Vásquez, Momar Ndao, Mehudy Medina, Marcelo Gottschalk, Mariolga Berrizbietia |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Chagas disease Canada Trypanosoma cruzi Antibodies Protozoan Antigens Protozoan Immunofluorescence Sensitivity and Specificity Microbiology Immunoenzyme Techniques Antigen parasitic diseases medicine Animals Humans Chagas Disease Amastigote biology medicine.diagnostic_test Kinetoplastida medicine.disease biology.organism_classification Venezuela Virology Protozoa Parasitology Trypanosomiasis |
| Popis: | Three enzyme immunoassays (EIAs) for diagnosis of Chagas' disease were developed with fixed forms of Trypanosoma cruzi using a panel of 435 sera from the following groups: Venezuelan subjects positive by immunofluorescence ( n = 70), Venezuelan healthy controls ( n = 85), healthy Canadians ( n = 166), and subjects with other parasitic diseases ( n = 114). All assays achieved 100% sensitivity and reasonable specificity for amastigotes (97.6%), epimastigotes (98.3%), and trypomastigotes (99.3%). The fixed-trypomastigote assay was stable over 4 months at 4°C and room temperature. These data suggest that a fixed-trypomastigote EIA may be a suitable candidate for blood bank screening. |
| Databáze: | OpenAIRE |
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