The binding of steroid hormones to degranulated microsomes from rat-liver endoplasmic reticulum
| Autor: | Carol A. Blyth, Brian R. Rabin, Anne Roobol, M.B. Cooper |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Receptors Cell Surface Biology Acetates Cell Fractionation Endoplasmic Reticulum Tritium Biochemistry Steroid Radioligand Assay Structure-Activity Relationship Sex Factors Polysome Internal medicine medicine Animals Testosterone Binding site Edetic Acid Binding Sites Estradiol Endoplasmic reticulum Proteins Rats Membrane Endocrinology Polyribosomes Microsome Microsomes Liver RNA Female Cell fractionation |
| Zdroj: | European journal of biochemistry. 29(2) |
| ISSN: | 0014-2956 |
| Popis: | 1 Smooth reticular membranes from male rat liver contain high affinity binding sites for oestradiol. Similar membranes from females contain analogous sites for testosterone. 2 Rough membranes from which polysomes have been removed by EDTA (degranulated membranes), contain no high affinity binding sites for either hormone. 3 Ethyl acetate extracts of degranulated membranes contain materials which block the high affinity binding sites on smooth membranes of the same but not the opposite sex. 4 These extracts promote the attachment of ribosomes to smooth membranes of the same but not the opposite sex. 5 The ethyl acetate extracts from male and female degranulated membranes contain substances which behave like oestradiol and testosterone, respectively. |
| Databáze: | OpenAIRE |
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