Expression of Estrogen Receptor-alpha in Nasal Polyps and the Effects of Dexamethasone on Estrogen Receptor-alpha Expression in RPMI 2650 Cells
| Autor: | Yoon-Jin Lee, Sang-Han Lee, Byoung Joon Baek, Won Woo Ban, Jae Yeop Jung |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cell Survival
Anti-Inflammatory Agents Tetrazolium Salts Estrogen receptor Apoptosis Turbinates Dexamethasone Cell Line 03 medical and health sciences Nasal Polyps 0302 clinical medicine Western blot Annexin Cell Viability medicine Humans MTT assay 030212 general & internal medicine Viability assay Fulvestrant bcl-2-Associated X Protein medicine.diagnostic_test Caspase 3 Chemistry Estrogen Receptor alpha Endoscopy Estrogen Receptor-α General Medicine Immunohistochemistry Molecular biology Thiazoles Proto-Oncogene Proteins c-bcl-2 Otorhinolaryngology RPMI 2650 Cells Keratins Original Article Nasal Polyp Estrogen receptor alpha medicine.drug |
| Zdroj: | Journal of Korean Medical Science |
| ISSN: | 1598-6357 1011-8934 |
| Popis: | Background Studies have reported that epithelial cell proliferation may be involved in the pathogenesis of nasal polyps (NPs). Estrogen receptor (ER)-α, one type of ER, is related to anti-inflammatory action and cell survival in certain tissues. In this study, we examined the presence or absence of ER-α in NPs and healthy inferior turbinate mucosae. We also investigated the effect of dexamethasone on ER-α expression, cell viability, and apoptosis in RPMI 2650 cells. Methods Immunohistochemical staining and Western blot analysis were conducted to determine the expression of ER-α in 15 NPs and 15 healthy inferior turbinate mucosae. After treating RPMI 2650 cells with dexamethasone, ER-α expression was analyzed using Western blot analysis and cell viability was determined using the MTT assay. Western blot analysis and annexin V-phycoerythrin (PE) staining were used to examine apoptotic cell death. Results Western blot analysis showed that ER-α expression was upregulated in 13 of the 15 NP tissues. Immunohistochemical staining for ER-α confirmed the results of the Western blot analysis. When RPMI 2650 cells were treated with dexamethasone, both ER-α expression and cell viability were decreased. Furthermore, the treatment of RPMI 2650 cells with dexamethasone increased apoptotic cell death, as shown by increased levels of BAX and cleaved caspase-3, decreased levels of Bcl-2, and an increased percentage of positive annexin V-PE stained cells. Conclusion ER-α expression was higher in NPs than in healthy inferior turbinate mucosae. When RPMI 2650 cells were treated with dexamethasone, ER-α expression was downregulated, cell viability decreased, and apoptosis increased. The decreased cell viability may be related, at least in part, to the decreased ER-α protein levels, which likely contributed to the induction of apoptotic cell death in RPMI 2650 cells. Graphical Abstract |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|