Safety and protective efficacy of porcine reproductive and respiratory syndrome recombinant virus vaccines in young pigs

Autor: I.F.A. van der Linden, Monique H. Verheije, E.A. de Boer-Luijtze, P.A. van Rijn, Johanna Jacoba Maria Meulenberg, P.J.G.M. Steverink, J.M.A. Pol, M. V. Kroese
Jazyk: angličtina
Rok vydání: 2003
Předmět:
ID - Infectieziekten
Time Factors
north-american
syndrome prrs virus
viruses
monoclonal-antibodies
Porcine Reproductive and Respiratory Syndrome
Heterologous
Virulence
Recombinant virus
nucleocapsid protein-n
Virus
law.invention
Arterivirus
strains
CIDC - Division Virology
Nidovirales
law
Macrophages
Alveolar
Animals
Porcine respiratory and reproductive syndrome virus
lelystad virus
genome
Cells
Cultured
DNA Primers
Vaccines
Synthetic
Base Sequence
General Veterinary
General Immunology and Microbiology
biology
Reverse Transcriptase Polymerase Chain Reaction
CIDC - Divisie Virologie
Public Health
Environmental and Occupational Health
structural proteins
Viral Vaccines
swine
biology.organism_classification
Porcine reproductive and respiratory syndrome virus
Virology
arteriviruses
Infectious Diseases
Amino Acid Substitution
Recombinant DNA
Wageningen Business Generator
Molecular Medicine
Safety
Zdroj: Vaccine, 21(19-20), 2556-2563
Vaccine 21 (2003) 19-20
ISSN: 0264-410X
Popis: Three porcine reproductive and respiratory syndrome virus (PRRSV) recombinants, generated by mutagenesis of an infectious cDNA clone of the Lelystad virus (LV) isolate, were tested for their safety and protective efficacy as potential PRRSV vaccines in pigs. Recombinant vABV688 contains two amino acid substitutions in the minor structural protein GP(2) resulting in improved growth on cell line CL2621; in recombinant vABV707 the region encoding the ectodomain of the major unglycosylated membrane protein M has been replaced by that of the murine lactate dehydrogenase-elevating arterivirus; recombinant vABV746 lacks the six C-terminal amino acids of the nucleocapsid protein N. First, we determined the safety of these recombinant viruses by monitoring the stability of the introduced mutations in 8-week-old pigs. We showed that the introduced genomic mutations were maintained throughout the viraemic period. Second, the protective efficacy of immunization with the recombinant viruses against challenge with a homologous and a heterologous PRRSV strain was determined in two pigs and compared with the efficacy of vABV437, a virus derived from the parental LV cDNA. The viraemia in pigs immunized with the recombinant viruses was reduced compared to pigs immunized with vABV437. In addition, the length of viraemia was reduced in the sentinel pigs that were introduced into the groups immunized with vABV746, vABV688, and vABV707, however, all of the sentinel pigs became infected. Pigs immunized with vABV707 and vABV437 were protected against challenge with homologous virus LV-Ter Huurne and transmission of the latter virus. None of the immunized pigs were protected against heterologous challenge with the virulent US isolate SDSU#73, but the vABV707- and vABV746-immunized pigs were protected against transmission of this virus from challenged pigs. In conclusion, the obtained viral recombinants are interesting candidates to be further explored for their use as vaccines against PRRSV.
Databáze: OpenAIRE
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