The design and analysis of a homeotic response element
| Autor: | Jenny J. Brookman, Graham Sproat, Simon E. Aspland, Lesley Clayton, Robert A. H. White |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Homeodomain Proteins
Genetics Embryology animal structures Base pair fungi Nuclear Proteins Biology Response Elements DNA-binding protein DNA-Binding Proteins Repressor Proteins A-site Homeotic selector gene embryonic structures Animals Drosophila Proteins Insect Proteins Drosophila Binding site Hox gene Homeotic gene Ultrabithorax Transcription Factors Developmental Biology |
| Zdroj: | Mechanisms of Development. 91:217-226 |
| ISSN: | 0925-4773 |
| DOI: | 10.1016/s0925-4773(99)00306-8 |
| Popis: | We have shown that the 26 bp bx1 element from the regulatory region of Distal-less is capable of imposing control by the homeotic genes Ultrabithorax and abdominal-A on a general epidermal activator in Drosophila . This provides us with an assay to analyze the sequence requirements for specific repression by these Hox genes. Both the core Hox binding site, 5′-TAAT, and the adjacent EXD 5′-TGAT core site are required for repression by Ultrabithorax and abdominal-A . The Distal-less bx1 site thus fits with the model of Hox protein binding specificity based on the consensus PBX/HOX-family site TGATNNAT[g/t][g/a], where the key elements of binding specificity are proposed to lie in the two base pairs following the TGAT. A single base pair deletion in the bx1 sequence generates a site, bx1:A − mut, that on the consensus PBX/HOX model would be expected to be regulated by the Deformed Hox gene. We observed, however, that the bx1:A − mut site was regulated predominantly by Sex combs reduced , Ultrabithorax and abdominal-A . The analysis of this site indicates that the specificity of action of Hox proteins may depend not only on selective DNA binding but also on specific post-binding interactions. |
| Databáze: | OpenAIRE |
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